Overview

  • Product nameAnti-PKC theta antibody
    See all PKC theta primary antibodies
  • Description
    Rabbit polyclonal to PKC theta
  • SpecificityThis antibody is specific for PKC theta.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthesized non-phosphopeptide derived from human PKC theta around the phosphorylation site of serine 676 (R-L-SP-F-A).

  • Positive control
    • JK cells

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab47471 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa).
ELISA 1/20000.

Target

  • FunctionThis is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production.
    PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
  • Tissue specificitySkeletal muscle, megakaryoblastic cells and platelets.
  • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • DomainThe C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.
  • Post-translational
    modifications
    Autophosphorylation at Thr-219 is required for targeting to the TCR and cellular function of PKC upon antigen receptor ligation.
  • Information by UniProt
  • Database links
  • Alternative names
    • KPCT_HUMAN antibody
    • MGC126514 antibody
    • MGC141919 antibody
    • nPKC theta antibody
    • nPKC-theta antibody
    • nPKCtheta antibody
    • OTTHUMP00000043364 antibody
    • OTTHUMP00000043365 antibody
    • PKC 0 antibody
    • PKC0 antibody
    • Prkcq antibody
    • PRKCT antibody
    • Protein kinase C theta antibody
    • Protein kinase C theta type antibody
    • Protein Kinase Ctheta antibody
    see all

Anti-PKC theta antibody images

  • All lanes : Anti-PKC theta antibody (ab47471) at 1/500 dilution

    Lane 1 : JK cells treated with PMA (200nM, 30min)
    Lane 2 : JK cells treated with PMA (200nM, 30min) with synthetic peptide

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 82 kDa
    Observed band size : 82 kDa

References for Anti-PKC theta antibody (ab47471)

This product has been referenced in:
  • Aguiló JI  et al. Protein kinase C-theta is required for NK cell activation and in vivo control of tumor progression. J Immunol 182:1972-81 (2009). WB ; Mouse . Read more (PubMed: 19201850) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"