Recombinant
RabMAb

Anti-PKC theta antibody [EPR1487(2)] (ab110728)

Overview

  • Product name
    Anti-PKC theta antibody [EPR1487(2)]
    See all PKC theta primary antibodies
  • Description
    Rabbit monoclonal [EPR1487(2)] to PKC theta
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human PKC theta.

  • Positive control
    • Human platelet and Jurkat lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    A trial size is available to purchase for this antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab110728 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 82 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function
      This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production.
      PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
    • Tissue specificity
      Skeletal muscle, megakaryoblastic cells and platelets.
    • Sequence similarities
      Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 1 C2 domain.
      Contains 2 phorbol-ester/DAG-type zinc fingers.
      Contains 1 protein kinase domain.
    • Domain
      The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.
    • Post-translational
      modifications
      Autophosphorylation at Thr-219 is required for targeting to the TCR and cellular function of PKC upon antigen receptor ligation.
    • Information by UniProt
    • Database links
    • Alternative names
      • KPCT_HUMAN antibody
      • MGC126514 antibody
      • MGC141919 antibody
      • nPKC theta antibody
      • nPKC-theta antibody
      • nPKCtheta antibody
      • OTTHUMP00000043364 antibody
      • OTTHUMP00000043365 antibody
      • PKC 0 antibody
      • PKC0 antibody
      • Prkcq antibody
      • PRKCT antibody
      • Protein kinase C theta antibody
      • Protein kinase C theta type antibody
      • Protein Kinase Ctheta antibody
      see all

    Images

    • All lanes : Anti-PKC theta antibody [EPR1487(2)] (ab110728) at 1/1000 dilution

      Lane 1 : Human platelet lysate
      Lane 2 : Jurkat lysate

      Lysates/proteins at 10 µg/ml per lane.


      Predicted band size : 82 kDa

    References

    This product has been referenced in:
    • Byerly J  et al. PRKCQ promotes oncogenic growth and anoikis resistance of a subset of triple-negative breast cancer cells. Breast Cancer Res 18:95 (2016). Read more (PubMed: 27663795) »
    • Bracamontes CG  et al. The serum protein profile of early parity which induces protection against breast cancer. Oncotarget 7:82538-82553 (2016). WB ; Human . Read more (PubMed: 27769065) »

    See all 2 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab110728.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up