Recombinant
RabMAb

Anti-PKC theta antibody [EPR1488Y] (ab52494)

Overview

  • Product name
    Anti-PKC theta antibody [EPR1488Y]
    See all PKC theta primary antibodies
  • Description
    Rabbit monoclonal [EPR1488Y] to PKC theta
  • Specificity
    This antibody detects both phosphorylated (pT538) and unphosphorylated PKC theta
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: ICC or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human PKC theta.

  • Positive control
    • Jurkat cell lysate
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab52494 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).
  • Application notes
    Is unsuitable for ICC or IHC-P.
  • Target

    • Function
      This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production.
      PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
    • Tissue specificity
      Skeletal muscle, megakaryoblastic cells and platelets.
    • Sequence similarities
      Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 1 C2 domain.
      Contains 2 phorbol-ester/DAG-type zinc fingers.
      Contains 1 protein kinase domain.
    • Domain
      The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.
    • Post-translational
      modifications
      Autophosphorylation at Thr-219 is required for targeting to the TCR and cellular function of PKC upon antigen receptor ligation.
    • Information by UniProt
    • Database links
    • Alternative names
      • KPCT_HUMAN antibody
      • MGC126514 antibody
      • MGC141919 antibody
      • nPKC theta antibody
      • nPKC-theta antibody
      • nPKCtheta antibody
      • OTTHUMP00000043364 antibody
      • OTTHUMP00000043365 antibody
      • PKC 0 antibody
      • PKC0 antibody
      • Prkcq antibody
      • PRKCT antibody
      • Protein kinase C theta antibody
      • Protein kinase C theta type antibody
      • Protein Kinase Ctheta antibody
      see all

    Images

    • All lanes : Anti-PKC theta antibody [EPR1488Y] (ab52494) at 1/1000 dilution (purified)

      Lane 1 : Rat brain lysate
      Lane 2 : Rat spleen lysate
      Lane 3 : C2C12 whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/20000 dilution

      Predicted band size : 85 kDa
      Observed band size : 78 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Anti-PKC theta antibody [EPR1488Y] (ab52494) at 1/1000 dilution (purified) + Jurkat whole cell lysate at 10 µg

      Secondary
      Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/20000 dilution

      Predicted band size : 85 kDa
      Observed band size : 78 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Anti-PKC theta antibody [EPR1488Y] (ab52494) at 1/500 dilution (unpurified) + Jurkat cell lysate at 10 µg

      Secondary
      HRP labeled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 85 kDa
      Observed band size : 85 kDa

    References

    ab52494 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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