Anti-PKC theta (phospho S676) antibody (ab47384)


  • Product nameAnti-PKC theta (phospho S676) antibody
    See all PKC theta primary antibodies
  • Description
    Rabbit polyclonal to PKC theta (phospho S676)
  • SpecificityDetects endogenous levels of PKC theta only when phosphorylated at serine 676.
  • Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    The antiserum was produced against synthesized phosphopeptide derived from human PKC theta around the phosphorylation site of serine 676 (R-L-SP-F-A).

  • Positive control
    • JK cells, human breast carcinoma tissue


Associated products


Our Abpromise guarantee covers the use of ab47384 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/4000.


  • FunctionThis is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production.
    PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
  • Tissue specificitySkeletal muscle, megakaryoblastic cells and platelets.
  • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • DomainThe C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.
  • Post-translational
    Autophosphorylation at Thr-219 is required for targeting to the TCR and cellular function of PKC upon antigen receptor ligation.
  • Information by UniProt
  • Database links
  • Alternative names
    • KPCT_HUMAN antibody
    • MGC126514 antibody
    • MGC141919 antibody
    • nPKC theta antibody
    • nPKC-theta antibody
    • nPKCtheta antibody
    • OTTHUMP00000043364 antibody
    • OTTHUMP00000043365 antibody
    • PKC 0 antibody
    • PKC0 antibody
    • Prkcq antibody
    • PRKCT antibody
    • Protein kinase C theta antibody
    • Protein kinase C theta type antibody
    • Protein Kinase Ctheta antibody
    see all

Anti-PKC theta (phospho S676) antibody images

  • All lanes : Anti-PKC theta (phospho S676) antibody (ab47384)

    Lane 1 : JK cell lysate
    Lane 2 : Lysate of JK cells treated with PMA (200nM, 30min)

    Predicted band size : 82 kDa
    Observed band size : 82 kDa
  • Ab47384 at 1/50 dilution staining paraffin-embedded human breast carcinoma tissue.

References for Anti-PKC theta (phospho S676) antibody (ab47384)

ab47384 has not yet been referenced specifically in any publications.

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