Recombinant Anti-PKC theta/PRKCQ (phospho T538) antibody [F4H4L1] (ab203565)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [F4H4L1] to PKC theta/PRKCQ (phospho T538)
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-PKC theta/PRKCQ (phospho T538) antibody [F4H4L1]
See all PKC theta/PRKCQ primary antibodies -
Description
Rabbit monoclonal [F4H4L1] to PKC theta/PRKCQ (phospho T538) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Human PKC theta/PRKCQ aa 500-600 (phospho T538).
Database link: Q04759 -
Positive control
- Jurkat cells and lysates stimulated with PMA; HeLa cells; Human breast carcinoma tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
F4H4L1 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab203565 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 82 kDa.
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IHC-P |
Use a concentration of 4 - 6 µg/ml.
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Flow Cyt |
Use a concentration of 8 - 12 µg/ml.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 82 kDa. |
IHC-P
Use a concentration of 4 - 6 µg/ml. |
Flow Cyt
Use a concentration of 8 - 12 µg/ml. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production.
PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. -
Tissue specificity
Skeletal muscle, megakaryoblastic cells and platelets. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain. -
Domain
The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain. -
Post-translational
modificationsAutophosphorylation at Thr-219 is required for targeting to the TCR and cellular function of PKC upon antigen receptor ligation. - Information by UniProt
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Database links
- Entrez Gene: 5588 Human
- Entrez Gene: 18761 Mouse
- Entrez Gene: 85420 Rat
- Omim: 600448 Human
- SwissProt: Q04759 Human
- SwissProt: Q02111 Mouse
- SwissProt: Q9WTQ0 Rat
- Unigene: 498570 Human
see all -
Alternative names
- KPCT_HUMAN antibody
- MGC126514 antibody
- MGC141919 antibody
see all
Images
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All lanes : Anti-PKC theta/PRKCQ (phospho T538) antibody [F4H4L1] (ab203565) at 3 µg/ml
Lane 1 : Jurkat lysates stimulated with 100 ng/mL PMA for 1 hour
Lane 2 : Jurkat lysates stimulated with 100 ng/mL PMA for 1 hour with Immunogen phosphopeptide
Lane 3 : Jurkat lysates stimulated with 100 ng/mL PMA for 1 hour with Non-phosphopeptide
Predicted band size: 82 kDa -
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast carcinoma tissue labeling PKC theta/PRKCQ (phospho T538) with ab203565 at 5 µg/mL followed by DAB staining. Magnification: 20x.
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Flow cytometric analysis of Jurkat cells (incubated with 100 μM PMA for 1 hour prior to being fixed and permeabilized) labeling PKC theta/PRKCQ (phospho T538 with ab203565 at 0.1 μg followed by Alexa Fluor® 488 goat anti-rabbit IgG (grey). Pre-incubation with the immunogenic phosphopeptide decreased the signal (red), whereas incubation with the non-phosphopeptide did not (blue).
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab203565 has not yet been referenced specifically in any publications.