Overview

  • Product nameAnti-PKC zeta antibody
    See all PKC zeta primary antibodies
  • Description
    Rabbit polyclonal to PKC zeta
  • Tested applicationsSuitable for: WB, ELISA, IHC-Fr, ICC, IPmore details
  • Species reactivity
    Reacts with: Rat
    Predicted to work with: all Mammals
  • Immunogen

    Synthetic peptide:

    EYINPLLLSAEESV

    , corresponding to amino acids 579-592 of Rat PKC zeta.

  • Positive control
    • Rat brain and myeloid tissues.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.05% Sodium Azide
  • PurityWhole antiserum
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab4137 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250. Predicted molecular weight: 71 kDa. (using horseradish peroxidase or alkaline phosphatase).
ELISA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC 1/250. Frozen. (using horseradish peroxidase or alkaline phosphatase).
IP 1/300. PubMed: 19028678

Target

  • FunctionThis is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Phosphorylates 'Ser-311' of RELA subunit of NF-kappa-B.
    PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Subunit of a quaternary complex that plays a central role in epithelial cell polarization.
  • Tissue specificityExpressed in brain, and to a lesser extent in lung, kidney and testis.
  • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 OPR domain.
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 protein kinase domain.
  • DomainThe OPR domain mediates mutually exclusive interactions with SQSTM1 and PARD6B.
    The C1 domain does not bind the diacylglycerol (DAG).
  • Post-translational
    modifications
    CDH5 is required for its phosphorylation at Thr-410.
  • Cellular localizationCytoplasm. Endosome. Cell junction. In the retina, localizes in the terminals of the rod bipolar cells (By similarity). Associates with endosomes. Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction.
  • Information by UniProt
  • Database links
  • Alternative names
    • 14-3-3-zetaisoform antibody
    • AI098070 antibody
    • aPKCzeta antibody
    • C80388 antibody
    • EC 2.7.11.13 antibody
    • KPCZ_HUMAN antibody
    • nPKC zeta antibody
    • nPKC-zeta antibody
    • OTTHUMP00000001368 antibody
    • OTTHUMP00000044160 antibody
    • PKC 2 antibody
    • PKC ZETA antibody
    • PKC2 antibody
    • Pkcz antibody
    • PKCZETA antibody
    • PKM-zeta, included antibody
    • PRKCZ antibody
    • Protein kinase C zeta antibody
    • Protein kinase C zeta form antibody
    • Protein kinase C zeta type antibody
    • r14-3-3 antibody
    • R74924 antibody
    • zetaPKC antibody
    see all

Anti-PKC zeta antibody images



  • Predicted band size : 71 kDa

    Image from Barros SA et al, J Biol Chem. 2009 Jan 30;284(5):2672-9. Epub 2008 Nov 21, Fig 2.

References for Anti-PKC zeta antibody (ab4137)

This product has been referenced in:
  • Barros SA  et al. Activation of protein kinase Czeta increases OAT1 (SLC22A6)- and OAT3 (SLC22A8)-mediated transport. J Biol Chem 284:2672-9 (2009). WB, IP ; Rat . Read more (PubMed: 19028678) »
  • Ort T  et al. Alterations in soleus muscle gene expression associated with a metabolic endpoint following exercise training by lean and obese Zucker rats. Physiol Genomics 29:302-11 (2007). Read more (PubMed: 17284668) »

See all 2 Publications for this product

Product Wall

Thank you for your inquiry. I have conducted a search for you and found the following antibodies: ab43176 - Rabbit polyclonal to PKC zeta ab4137 - Chicken polyclonal to ATP Synthase beta ab51157 - Rabbit polyclonal to PKC zeta ab59412 - Rabb...

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Thank you for your patience and for providing details regarding your protocol. Ab4137 was characterized for use in Western blotting with rat brain and myeloid tissues. This antibody has not yet been tested for cross-reactivity with human samples. I wou...

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Thank you for your enquiry. Did you try increasing the concentration of the primary to 1:100? Please let me know if that improved your results. What specifically are the rat samples that you are using? Can you tell me as well, how did you store the ant...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"