Overview

  • Product name
  • Description
    Rabbit polyclonal to PKC zeta
  • Specificity
    ab51157 detects endogenous levels of total PKC zeta protein.
  • Tested applications
    Suitable for: ICC/IF, ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic non-phosphopeptide derived from human PKC zeta around the phosphorylation site of threonine 410 (T-S-TP-F-C).

  • Positive control
    • human lung carcinoma tissue, extracts from 3T3 cells treated with PMA

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab51157 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 5 µg/ml.
ELISA 1/20000.
IHC-P Use at an assay dependent concentration.
WB 1/500 - 1/1000. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa).

Target

  • Function
    This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Phosphorylates 'Ser-311' of RELA subunit of NF-kappa-B.
    PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Subunit of a quaternary complex that plays a central role in epithelial cell polarization.
  • Tissue specificity
    Expressed in brain, and to a lesser extent in lung, kidney and testis.
  • Sequence similarities
    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 OPR domain.
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 protein kinase domain.
  • Domain
    The OPR domain mediates mutually exclusive interactions with SQSTM1 and PARD6B.
    The C1 domain does not bind the diacylglycerol (DAG).
  • Post-translational
    modifications
    CDH5 is required for its phosphorylation at Thr-410.
  • Cellular localization
    Cytoplasm. Endosome. Cell junction. In the retina, localizes in the terminals of the rod bipolar cells (By similarity). Associates with endosomes. Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction.
  • Information by UniProt
  • Database links
  • Alternative names
    • 14-3-3-zetaisoform antibody
    • AI098070 antibody
    • aPKCzeta antibody
    • C80388 antibody
    • EC 2.7.11.13 antibody
    • KPCZ_HUMAN antibody
    • nPKC zeta antibody
    • nPKC-zeta antibody
    • OTTHUMP00000001368 antibody
    • OTTHUMP00000044160 antibody
    • PKC 2 antibody
    • PKC ZETA antibody
    • PKC2 antibody
    • Pkcz antibody
    • PKCZETA antibody
    • PKM-zeta, included antibody
    • PRKCZ antibody
    • Protein kinase C zeta antibody
    • Protein kinase C zeta form antibody
    • Protein kinase C zeta type antibody
    • r14-3-3 antibody
    • R74924 antibody
    • zetaPKC antibody
    see all

Anti-PKC zeta antibody images

  • All lanes : Anti-PKC zeta antibody (ab51157) at 1/500 dilution

    Lane 1 : extracts from 3T3 cells treated with 125ng/ml PMA for 30min, without immunizing peptide
    Lane 2 : extracts from 3T3 cells treated with 125ng/ml PMA for 30min, with immunizing peptide


    Predicted band size : 67 kDa
    Observed band size : 67 kDa
  • Immunohistochemical analysis of PKC zeta expression in paraffin-embedded human lung carcinoma tissue using 1/50 ab51157. Left: untreated sample. Right: sample treated with immunizing peptide (negative control).
  • ICC/IF image of ab51157 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51157, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-PKC zeta antibody (ab51157)

This product has been referenced in:
  • Christian F  et al. PKA phosphorylation of p62/SQSTM1 regulates PB1 domain interaction partner binding. Biochim Biophys Acta 1843:2765-74 (2014). Read more (PubMed: 25110345) »
  • Jagan IC  et al. PTEN phosphatase-independent maintenance of glandular morphology in a predictive colorectal cancer model system. Neoplasia 15:1218-30 (2013). Human . Read more (PubMed: 24348097) »

See all 3 Publications for this product

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