Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (ab76129)

Overview

  • Product nameAnti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y]
  • Description
    Rabbit monoclonal [EP1491Y] to PKC zeta (phospho T410) + PKC lambda (phospho T412)
  • Specificityab76129 detects PKC zeta and PKC lamda phosphorylated at T410/412 respectively.
  • Tested applicationsSuitable for: IHC-P, WBmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to PKC zeta.

  • Positive control
    • 293 cell lysate, human brain tissue; HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

     

Properties

Applications

Our Abpromise guarantee covers the use of ab76129 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

WB 1/20000. Predicted molecular weight: 68 kDa.

For unpurified, use 1/1000 - 1/2000.

  • Application notesIs unsuitable for IP.
  • Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] images

    • Immunohistochemical staining of paraffin embedded rat cerebral cortex with purified ab76129 at a working dilution of 1 in 100. The secondary antibody used is a HRP goat anti-rabbit IgG (ab97051), used at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    • Immunohistochemical staining of paraffin embedded mouse kidney with purified ab76129 at a working dilution of 1 in 100. The secondary antibody used is a HRP goat anti-rabbit IgG (ab97051), used at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    • Immunohistochemical staining of paraffin embedded human endometrium with purified ab76129 at a working dilution of 1 in 100. The secondary antibody used is a HRP goat anti-rabbit IgG (ab97051), used at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    • Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (ab76129) at 1/20000 dilution (purified) + Mouse lung tissue lysate at 10 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size : 68 kDa
      Observed band size : 74 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (ab76129) at 1/20000 dilution (purified)

      Lane 1 : Untreated HeLa cells
      Lane 2 : HeLa cells treated with alkaline phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size : 68 kDa
      Additional bands at : 74 kDa. We are unsure as to the identity of these extra bands.

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Immunohistochemical staining of PKC zeta in human brain with unpurified ab76129 at 1/100 dilution.

    • All lanes : Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (ab76129) at 1/2000 dilution (unpurified)

      Lane 1 : 293 cell lysates, untreated
      Lane 2 : 293 cell lysates, treated with AP

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit-HRP at 1/1000 dilution

      Predicted band size : 68 kDa
      Observed band size : 68 kDa
      Additional bands at : 165 kDa,170 kDa. We are unsure as to the identity of these extra bands.

    References for Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (ab76129)

    This product has been referenced in:
    • Raychaudhuri S MicroRNAs Overexpressed in Growth-Restricted Rat Skeletal Muscles Regulate the Glucose Transport in Cell Culture Targeting Central TGF-ß Factor SMAD4. PLoS One 7:e34596 (2012). Read more (PubMed: 22506032) »

    See 1 Publication for this product

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