• Product nameAnti-PKC zeta (phospho T560) antibody
    See all PKC zeta primary antibodies
  • Description
    Rabbit polyclonal to PKC zeta (phospho T560)
  • SpecificityDetects endogenous levels of PKC? only when phosphorylated at threonine 560.
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic phosphopeptide derived from human PKCζ around the phosphorylation site of threonine 560 (Q-L-TP-P-D).

  • Positive control
    • Brain tissue,COS7 cells.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAffinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products


Our Abpromise guarantee covers the use of ab59412 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
ELISA 1/20000.
IHC-P 1/50 - 1/100.
IHC-Fr Use at an assay dependent concentration. PubMed: 23675446


  • FunctionThis is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Phosphorylates 'Ser-311' of RELA subunit of NF-kappa-B.
    PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Subunit of a quaternary complex that plays a central role in epithelial cell polarization.
  • Tissue specificityExpressed in brain, and to a lesser extent in lung, kidney and testis.
  • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 OPR domain.
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 protein kinase domain.
  • DomainThe OPR domain mediates mutually exclusive interactions with SQSTM1 and PARD6B.
    The C1 domain does not bind the diacylglycerol (DAG).
  • Post-translational
    CDH5 is required for its phosphorylation at Thr-410.
  • Cellular localizationCytoplasm. Endosome. Cell junction. In the retina, localizes in the terminals of the rod bipolar cells (By similarity). Associates with endosomes. Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction.
  • Information by UniProt
  • Database links
  • Alternative names
    • 14-3-3-zetaisoform antibody
    • AI098070 antibody
    • aPKCzeta antibody
    • C80388 antibody
    • EC antibody
    • KPCZ_HUMAN antibody
    • nPKC zeta antibody
    • nPKC-zeta antibody
    • OTTHUMP00000001368 antibody
    • OTTHUMP00000044160 antibody
    • PKC 2 antibody
    • PKC ZETA antibody
    • PKC2 antibody
    • Pkcz antibody
    • PKCZETA antibody
    • PKM-zeta, included antibody
    • PRKCZ antibody
    • Protein kinase C zeta antibody
    • Protein kinase C zeta form antibody
    • Protein kinase C zeta type antibody
    • r14-3-3 antibody
    • R74924 antibody
    • zetaPKC antibody
    see all

Anti-PKC zeta (phospho T560) antibody images

  • All lanes : Anti-PKC zeta (phospho T560) antibody (ab59412) at 1/500 dilution

    Lane 1 : Extracts from COS7 cells treated with PMA (125ng/ml, 30mins)
    Lane 2 : Extracts from COS7 cells treated with PMA (125ng/ml, 30mins) with phosphopeptide

    Predicted band size : 68 kDa
    Observed band size : 68 kDa
  • Immunohistochemical analysis of paraffin embedded human brain gliomas using ab59412 at 1/50 dilution

  • Immunohistochemical analysis of paraffin embedded human brain tissue using ab59412 at 1/50 dilution, in the presence (right) and absence (left) of immunising phosphopeptide.
  • ELISA showing that ab59412 (1:20000) detects endogenous levels of PKC zeta only when phosphorylated at Thr560.

References for Anti-PKC zeta (phospho T560) antibody (ab59412)

This product has been referenced in:

See all 3 Publications for this product

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