Overview

  • Product name
    Anti-PKHG1 antibody
    See all PKHG1 primary antibodies
  • Description
    Rabbit polyclonal to PKHG1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    SLKRAKRSTF LGLEADFVCC DSLRPFVSQD SLQLSEDEAP YHQATPDHGY LSLLYDSPSG NLSMPHKPVS DKLSEEVDEI WNDLENY, corresponding to amino acids 759-845 of Human PKHG1

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • Human duodenum tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121979 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Sequence similarities
    Contains 1 DH (DBL-homology) domain.
    Contains 1 PH domain.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • Database links
    • Alternative names
      • ARHGEF41 antibody
      • PKHG1_HUMAN antibody
      • Pleckstrin homology domain containing family G (with RhoGef domain) member 1 antibody
      • Pleckstrin homology domain containing family G member 1 antibody
      • Pleckstrin homology domain-containing family G member 1 antibody
      • PLEKHG1 antibody
      • RP11-25C17.1 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-PKHG1 antibody (ab121979)
      Immunocytochemistry/ Immunofluorescence - Anti-PKHG1 antibody (ab121979)
      Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus. Recommended concentration of ab121979 1-4 µg/ml. Cells treated with PFA/Triton X-100.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKHG1 antibody (ab121979)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKHG1 antibody (ab121979)
      ab121979, at a 1/20 dilution, staining PKHG1 in paraffin-embedded Human duodenum, by Immunohistochemistry.

    References

    ab121979 has not yet been referenced specifically in any publications.

    Publishing research using ab121979? Please let us know so that we can cite the reference in this datasheet.

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