antigen sequence: RNAIHDILVS SASGNSNELA LKLAGLDINK TEGEEDAQRS STEQSGEAQG EAAKSES, corresponding to amino acids 20-76 of Human PKI-alpha.
Our Abpromise guarantee covers the use of ab122816 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
ab122816 has not yet been referenced specifically in any publications.