Anti-PKI-alpha antibody (ab122816)

Overview

  • Product name
    Anti-PKI-alpha antibody
  • Description
    Rabbit polyclonal to PKI-alpha
  • Tested applications
    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    antigen sequence: RNAIHDILVS SASGNSNELA LKLAGLDINK TEGEEDAQRS STEQSGEAQG EAAKSES, corresponding to amino acids 20-76 of Human PKI-alpha.

  • Positive control
    • Human pancreas tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab122816 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

  • Function
    Extremely potent competitive inhibitor of cAMP-dependent protein kinase activity, this protein interacts with the catalytic subunit of the enzyme after the cAMP-induced dissociation of its regulatory chains.
  • Sequence similarities
    Belongs to the PKI family.
  • Information by UniProt
  • Database links
  • Alternative names
    • cAMP-dependent protein kinase inhibitor alpha antibody
    • cAMP-dependent protein kinase inhibitor antibody
    • IPKA_HUMAN antibody
    • muscle/brain isoform antibody
    • PKI-alpha antibody
    • Pkia antibody
    • PRKACN1 antibody
    • Protein kinase (cAMP-dependent, catalytic) inhibitor alpha antibody
    see all

Images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoplasm. Recommended concentration of ab122816 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122816, at 1/500 dilution, staining PKIA in paraffin-embedded Human pancreas tissue by Immunohistochemistry.

References

ab122816 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (brain)
Permeabilization
Yes - citrate buffer pH6
Specification
brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Mar 27 2017

Thank you for contacting us.

The anti-PKI-alpha antibody (ab122816) is predicted to react with the mouse PKI-alpha protein. As can be seen from the sequence alignment of the human and mouse proteins in the attachment to this email, the seque...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up