The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: Use at an assay dependent dilution.
WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 8 kDa.
This antibody has only been tested in WB against the recombinant protein used as immunogen. We have no data on the detection of endogenous protein.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevancePKIG is an extremely potent competitive inhibitor of cAMP-dependent protein kinase activity. It interacts with the catalytic subunit of the enzyme after the cAMP-induced dissociation of its regulatory chains.