Anti-PKLR antibody (ab125697)
Key features and details
- Rabbit polyclonal to PKLR
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PKLR antibody
See all PKLR primary antibodies -
Description
Rabbit polyclonal to PKLR -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment containing a sequence corresponding to a region within amino acids 178-510 of Human PKLR.(Uniprot:P30613)
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Positive control
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab125697 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 62 kDa.
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IHC-P |
1/100 - 1/500. Perfrom antigen retrieval using heat mediated 10mM Citrate buffer(pH6.0) or Tris-EDTA buffer(pH8.0).
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ICC/IF |
1/100 - 1/500.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 62 kDa. |
IHC-P
1/100 - 1/500. Perfrom antigen retrieval using heat mediated 10mM Citrate buffer(pH6.0) or Tris-EDTA buffer(pH8.0). |
ICC/IF
1/100 - 1/500. |
Target
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Function
Plays a key role in glycolysis. -
Pathway
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5. -
Involvement in disease
Defects in PKLR are the cause of pyruvate kinase hyperactivity (PKHYP) [MIM:102900]; also known as high red cell ATP syndrome. This autosomal dominant phenotype is characterized by increase of red blood cell ATP.
Defects in PKLR are the cause of pyruvate kinase deficiency of red cells (PKRD) [MIM:266200]. A frequent cause of hereditary non-spherocytic hemolytic anemia. Clinically, pyruvate kinase-deficient patients suffer from a highly variable degree of chronic hemolysis, ranging from severe neonatal jaundice and fatal anemia at birth, severe transfusion-dependent chronic hemolysis, moderate hemolysis with exacerbation during infection, to a fully compensated hemolysis without apparent anemia. -
Sequence similarities
Belongs to the pyruvate kinase family. - Information by UniProt
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Database links
- Entrez Gene: 5313 Human
- Omim: 609712 Human
- SwissProt: P30613 Human
- Unigene: 95990 Human
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Alternative names
- EC 2.7.1.40 antibody
- KPYR_HUMAN antibody
- L-PK antibody
see all
Images
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Anti-PKLR antibody (ab125697) at 1/1000 dilution + A549 cell lysate at 30 µg
Predicted band size: 62 kDa -
ab125697, at 1/250 dilution, staining PKLR in Formalin-fixed, Paraffin-embedded Human breast cancer tissue by Immunohistochemistry.
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ab125697, at 1/500 dilution, staining PKLR in Methanol-fixed HeLa cells by Immunocytochemistry/Immunofluorescence.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab125697 has not yet been referenced specifically in any publications.