Overview

  • Product nameAnti-PKLR antibody
    See all PKLR primary antibodies
  • Description
    Rabbit polyclonal to PKLR
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment containing a sequence corresponding to a region within amino acids 178-510 of Human PKLR.(Uniprot:P30613)

  • Positive control
    • A549 cell lysate, HeLa cell lysate, HepG2 cell lysate, HTC116 cell lysate and Human breast cancer tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab125697 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 62 kDa.
IHC-P 1/100 - 1/500. Perfrom antigen retrieval using heat mediated 10mM Citrate buffer(pH6.0) or Tris-EDTA buffer(pH8.0).
ICC/IF 1/100 - 1/500.

Target

  • FunctionPlays a key role in glycolysis.
  • PathwayCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
  • Involvement in diseaseDefects in PKLR are the cause of pyruvate kinase hyperactivity (PKHYP) [MIM:102900]; also known as high red cell ATP syndrome. This autosomal dominant phenotype is characterized by increase of red blood cell ATP.
    Defects in PKLR are the cause of pyruvate kinase deficiency of red cells (PKRD) [MIM:266200]. A frequent cause of hereditary non-spherocytic hemolytic anemia. Clinically, pyruvate kinase-deficient patients suffer from a highly variable degree of chronic hemolysis, ranging from severe neonatal jaundice and fatal anemia at birth, severe transfusion-dependent chronic hemolysis, moderate hemolysis with exacerbation during infection, to a fully compensated hemolysis without apparent anemia.
  • Sequence similaritiesBelongs to the pyruvate kinase family.
  • Information by UniProt
  • Database links
  • Alternative names
    • EC 2.7.1.40 antibody
    • KPYR_HUMAN antibody
    • L-PK antibody
    • Pk-1 antibody
    • PK1 antibody
    • PKL antibody
    • Pklg antibody
    • Pklr antibody
    • PKR antibody
    • PKRL antibody
    • Pyruvate kinase 1 antibody
    • Pyruvate kinase isozymes R/L antibody
    • Pyruvate kinase liver and blood cell antibody
    • Pyruvate kinase liver and RBC antibody
    • Pyruvate kinase liver and red blood cell antibody
    • Pyruvate kinase liver type antibody
    • Pyruvate kinase type L antibody
    • Pyruvate kinase, red cell type antibody
    • R type/L type pyruvate kinase antibody
    • R-PK antibody
    • R-type/L-type pyruvate kinase antibody
    • Red cell/liver pyruvate kinase antibody
    • RPK antibody
    see all

Anti-PKLR antibody images

  • ab125697, at 1/250 dilution, staining PKLR in Formalin-fixed, Paraffin-embedded Human breast cancer tissue by Immunohistochemistry.
  • ab125697, at 1/500 dilution, staining PKLR in Methanol-fixed HeLa cells by Immunocytochemistry/Immunofluorescence.
  • Anti-PKLR antibody (ab125697) at 1/1000 dilution + A549 cell lysate at 30 µg

    Predicted band size : 62 kDa

References for Anti-PKLR antibody (ab125697)

ab125697 has not yet been referenced specifically in any publications.

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