Anti-PKLR antibody (ab137787)
Key features and details
- Rabbit polyclonal to PKLR
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-PKLR antibody
See all PKLR primary antibodies -
Description
Rabbit polyclonal to PKLR -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 254-574 of Human PKLR
-
Positive control
- Jurkat, Raji, K562, THP-1 and NCI-H929 cell extracts; Mouse and Rat liver extracts
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137787 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 62 kDa.
|
Notes |
---|
WB
1/500 - 1/3000. Predicted molecular weight: 62 kDa. |
Target
-
Function
Plays a key role in glycolysis. -
Pathway
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5. -
Involvement in disease
Defects in PKLR are the cause of pyruvate kinase hyperactivity (PKHYP) [MIM:102900]; also known as high red cell ATP syndrome. This autosomal dominant phenotype is characterized by increase of red blood cell ATP.
Defects in PKLR are the cause of pyruvate kinase deficiency of red cells (PKRD) [MIM:266200]. A frequent cause of hereditary non-spherocytic hemolytic anemia. Clinically, pyruvate kinase-deficient patients suffer from a highly variable degree of chronic hemolysis, ranging from severe neonatal jaundice and fatal anemia at birth, severe transfusion-dependent chronic hemolysis, moderate hemolysis with exacerbation during infection, to a fully compensated hemolysis without apparent anemia. -
Sequence similarities
Belongs to the pyruvate kinase family. - Information by UniProt
-
Database links
- Entrez Gene: 5313 Human
- Entrez Gene: 18770 Mouse
- Entrez Gene: 24651 Rat
- Omim: 609712 Human
- SwissProt: P30613 Human
- SwissProt: P53657 Mouse
- SwissProt: P12928 Rat
- Unigene: 95990 Human
see all -
Alternative names
- EC 2.7.1.40 antibody
- KPYR_HUMAN antibody
- L-PK antibody
see all
Images
-
All lanes : Anti-PKLR antibody (ab137787) at 1/1000 dilution
Lane 1 : Jurkat cell extract
Lane 2 : Raji cell extract
Lane 3 : K562 cell extract
Lane 4 : THP-1 cell extract
Lane 5 : NCI-H929 cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 62 kDa
7.5% SDS PAGE -
Anti-PKLR antibody (ab137787) at 1/500 dilution + Mouse liver extract at 50 µg
Predicted band size: 62 kDa
7.5% SDS PAGE -
Anti-PKLR antibody (ab137787) at 1/500 dilution + Rat liver extract at 50 µg
Predicted band size: 62 kDa
7.5% SDS PAGE
Datasheets and documents
-
SDS download
-
Datasheet download
References (2)
ab137787 has been referenced in 2 publications.
- Yuan Q et al. Role of pyruvate kinase M2-mediated metabolic reprogramming during podocyte differentiation. Cell Death Dis 11:355 (2020). PubMed: 32393782
- Dixit D et al. Chaetocin-induced ROS-mediated apoptosis involves ATM-YAP1 axis and JNK-dependent inhibition of glucose metabolism. Cell Death Dis 5:e1212 (2014). WB ; Human . PubMed: 24810048