Overview

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab38240 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
IHC-P 1/50 - 1/100.
ELISA 1/1000.

Target

  • FunctionPlays a key role in glycolysis.
  • PathwayCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
  • Involvement in diseaseDefects in PKLR are the cause of pyruvate kinase hyperactivity (PKHYP) [MIM:102900]; also known as high red cell ATP syndrome. This autosomal dominant phenotype is characterized by increase of red blood cell ATP.
    Defects in PKLR are the cause of pyruvate kinase deficiency of red cells (PKRD) [MIM:266200]. A frequent cause of hereditary non-spherocytic hemolytic anemia. Clinically, pyruvate kinase-deficient patients suffer from a highly variable degree of chronic hemolysis, ranging from severe neonatal jaundice and fatal anemia at birth, severe transfusion-dependent chronic hemolysis, moderate hemolysis with exacerbation during infection, to a fully compensated hemolysis without apparent anemia.
  • Sequence similaritiesBelongs to the pyruvate kinase family.
  • Information by UniProt
  • Database links
  • Alternative names
    • EC 2.7.1.40 antibody
    • KPYR_HUMAN antibody
    • L-PK antibody
    • Pk-1 antibody
    • PK1 antibody
    • PKL antibody
    • Pklg antibody
    • Pklr antibody
    • PKR antibody
    • PKRL antibody
    • Pyruvate kinase 1 antibody
    • Pyruvate kinase isozymes R/L antibody
    • Pyruvate kinase liver and blood cell antibody
    • Pyruvate kinase liver and RBC antibody
    • Pyruvate kinase liver and red blood cell antibody
    • Pyruvate kinase liver type antibody
    • Pyruvate kinase type L antibody
    • Pyruvate kinase, red cell type antibody
    • R type/L type pyruvate kinase antibody
    • R-PK antibody
    • R-type/L-type pyruvate kinase antibody
    • Red cell/liver pyruvate kinase antibody
    • RPK antibody
    see all

Anti-PKLR antibody images

  • Anti-PKLR antibody (ab38240) at 1/100 dilution + NIH/3T3 cell lysate

    Predicted band size : 62 kDa
    Observed band size : 62 kDa
    Additional bands at : 30 kDa,50 kDa,90 kDa. We are unsure as to the identity of these extra bands.
  • Formalin-fixed and paraffin-embedded human breast cancer tissue staineded with ab38240 at a 1/50 dilution, using a peroxidase-conjugated secondary antibody, followed by AEC staining.

    This data demonstrates the suitability of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

References for Anti-PKLR antibody (ab38240)

This product has been referenced in:
  • Li YG  et al. 1-deoxynojirimycin inhibits glucose absorption and accelerates glucose metabolism in streptozotocin-induced diabetic mice. Sci Rep 3:1377 (2013). WB ; Mouse . Read more (PubMed: 23536174) »
  • Li YG  et al. Cooperative anti-diabetic effects of deoxynojirimycin-polysaccharide by inhibiting glucose absorption and modulating glucose metabolism in streptozotocin-induced diabetic mice. PLoS One 8:e65892 (2013). WB ; Mouse . Read more (PubMed: 23755289) »

See all 2 Publications for this product

Product Wall

Thank you for your enquiry. In answer to your question the sequence of the immunogen used to raise this antibody was SSLQLRSWVSKSQRD. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"