Overview

  • Product nameAnti-PKLR antibody
    See all PKLR primary antibodies
  • Description
    Mouse polyclonal to PKLR
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length Human PKLR protein, amino acids 1-574 (NP_000289.1)

  • Positive control
    • Human liver tissue lysate, PKLR transfected 293T cell lysate, Human kidney tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab89071 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
  • Application notesIHC-P: Use at a concentration of 3 µg/ml. Antigen retrieval is recommended: Place sample in 1X citrate buffer (pH 6.0) and microwave at 750W for 20 minutes, cool sample
    WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 62 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionPlays a key role in glycolysis.
    • PathwayCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
    • Involvement in diseaseDefects in PKLR are the cause of pyruvate kinase hyperactivity (PKHYP) [MIM:102900]; also known as high red cell ATP syndrome. This autosomal dominant phenotype is characterized by increase of red blood cell ATP.
      Defects in PKLR are the cause of pyruvate kinase deficiency of red cells (PKRD) [MIM:266200]. A frequent cause of hereditary non-spherocytic hemolytic anemia. Clinically, pyruvate kinase-deficient patients suffer from a highly variable degree of chronic hemolysis, ranging from severe neonatal jaundice and fatal anemia at birth, severe transfusion-dependent chronic hemolysis, moderate hemolysis with exacerbation during infection, to a fully compensated hemolysis without apparent anemia.
    • Sequence similaritiesBelongs to the pyruvate kinase family.
    • Information by UniProt
    • Database links
    • Alternative names
      • EC 2.7.1.40 antibody
      • KPYR_HUMAN antibody
      • L-PK antibody
      • Pk-1 antibody
      • PK1 antibody
      • PKL antibody
      • Pklg antibody
      • Pklr antibody
      • PKR antibody
      • PKRL antibody
      • Pyruvate kinase 1 antibody
      • Pyruvate kinase isozymes R/L antibody
      • Pyruvate kinase liver and blood cell antibody
      • Pyruvate kinase liver and RBC antibody
      • Pyruvate kinase liver and red blood cell antibody
      • Pyruvate kinase liver type antibody
      • Pyruvate kinase type L antibody
      • Pyruvate kinase, red cell type antibody
      • R type/L type pyruvate kinase antibody
      • R-PK antibody
      • R-type/L-type pyruvate kinase antibody
      • Red cell/liver pyruvate kinase antibody
      • RPK antibody
      see all

    Anti-PKLR antibody images

    • Anti-PKLR antibody (ab89071) at 1 µg/ml + Human liver tissue lysate at 50 µg

      Predicted band size : 62 kDa
      Observed band size : 62 kDa
    • All lanes : Anti-PKLR antibody (ab89071) at 1 µg/ml

      Lane 1 : PKLR transfected 293T cell lysate
      Lane 2 : Non-transfected lysate

      Lysates/proteins at 25 µg per lane.


      Predicted band size : 62 kDa
      Observed band size : 48 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
    • ab89071 at 3µg/ml staining PKLR in Human kidney by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue.

    References for Anti-PKLR antibody (ab89071)

    ab89071 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"