Anti-PKM2 antibody (ab79070)

Overview

  • Product name
  • Description
    Goat polyclonal to PKM2
  • Specificity
    The antibody is raised against pyruvate kinase isolated from rabbit muscle. Reactivity with specific PK (PKM1, PKLR) has not been assessed.
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Rabbit, Human
    Predicted to work with: Mammal
  • Immunogen

    Purified pyruvate kinase from Rabbit muscle.

  • Positive control
    • Uterus tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab79070 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml.

Target

  • Function
    Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
  • Tissue specificity
    Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells.
  • Pathway
    Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
  • Sequence similarities
    Belongs to the pyruvate kinase family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
    ISGylated.
  • Cellular localization
    Cytoplasm. Nucleus. Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic actvity.
  • Information by UniProt
  • Database links
  • Alternative names
    • CTHBP antibody
    • Cytosolic thyroid hormone binding protein antibody
    • Cytosolic thyroid hormone-binding protein antibody
    • KPYM_HUMAN antibody
    • MGC3932 antibody
    • OIP 3 antibody
    • OIP-3 antibody
    • OIP3 antibody
    • OPA interacting protein 3 antibody
    • Opa-interacting protein 3 antibody
    • p58 antibody
    • PK muscle type antibody
    • PK, muscle type antibody
    • PK2 antibody
    • PK3 antibody
    • PKM antibody
    • PKM2 antibody
    • pykm antibody
    • Pyruvate kinase 2/3 antibody
    • Pyruvate kinase 3 antibody
    • Pyruvate kinase isozymes M1/M2 antibody
    • Pyruvate kinase muscle antibody
    • Pyruvate kinase muscle isozyme antibody
    • pyruvate kinase PKM antibody
    • Pyruvate kinase, muscle 2 antibody
    • TCB antibody
    • THBP1 antibody
    • Thyroid hormone binding protein 1 antibody
    • Thyroid hormone binding protein cytosolic antibody
    • Thyroid hormone-binding protein 1 antibody
    • Tumor M2 PK antibody
    • Tumor M2-PK antibody
    see all

Images

  • ab79070, at 5µg/ml, staining PKM2 in uterus tissue by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.

References

ab79070 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10000µg/mL · Temperature: 25°C
Sample
Human Cell (Cholangiocarcinoma cell)
Specification
Cholangiocarcinoma cell
Permeabilization
Yes - 0.25% TritonX-100
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted May 26 2014



J’ai contacté le laboratoire est malheureusement l’épitope d’ab79070 n’a pas été déterminé.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (ECR 293 cell line)
Specification
ECR 293 cell line
Fixative
Formaldehyde
Permeabilization
Yes - 0.1% Triton X-100
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Dr. Ismail Hendaoui

Verified customer

Submitted Feb 04 2013

Merci pour votre appel de hier.

La laboratoire à pu me confirmer, que pour l'IP1 x 107 cellules ont été lysées et resuspendues en 0.2ml. Ensuite, ils ont ajouté de 0.5à 5 ul de cet anticorps et incub&...

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