• Product nameAnti-PKM2 antibody
    See all PKM2 primary antibodies
  • Description
    Rabbit polyclonal to PKM2
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human PKM2.

    (Peptide available as ab94378.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: HeLa; MEL-1; HepG2; MCF7; Caco 2; SHSY-5Y; Raji


Associated products


Our Abpromise guarantee covers the use of ab85555 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
ICC/IF Use a concentration of 5 µg/ml.


  • FunctionGlycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
  • Tissue specificitySpecifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells.
  • PathwayCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
  • Sequence similaritiesBelongs to the pyruvate kinase family.
  • Post-translational
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationCytoplasm. Nucleus. Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic actvity.
  • Information by UniProt
  • Database links
  • Alternative names
    • CTHBP antibody
    • Cytosolic thyroid hormone binding protein antibody
    • Cytosolic thyroid hormone-binding protein antibody
    • KPYM_HUMAN antibody
    • MGC3932 antibody
    • OIP 3 antibody
    • OIP-3 antibody
    • OIP3 antibody
    • OPA interacting protein 3 antibody
    • Opa-interacting protein 3 antibody
    • p58 antibody
    • PK muscle type antibody
    • PK, muscle type antibody
    • PK2 antibody
    • PK3 antibody
    • PKM antibody
    • PKM2 antibody
    • pykm antibody
    • Pyruvate kinase 2/3 antibody
    • Pyruvate kinase 3 antibody
    • Pyruvate kinase isozymes M1/M2 antibody
    • Pyruvate kinase muscle antibody
    • Pyruvate kinase muscle isozyme antibody
    • pyruvate kinase PKM antibody
    • Pyruvate kinase, muscle 2 antibody
    • TCB antibody
    • THBP1 antibody
    • Thyroid hormone binding protein 1 antibody
    • Thyroid hormone binding protein cytosolic antibody
    • Thyroid hormone-binding protein 1 antibody
    • Tumor M2 PK antibody
    • Tumor M2-PK antibody
    see all

Anti-PKM2 antibody images

  • All lanes : Anti-PKM2 antibody (ab85555) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : MEL-1 (Human embryonic stem cell, male cell line) Whole Cell Lysate (ab27198)
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 5 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
    Lane 6 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
    Lane 7 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 57 kDa
    Observed band size : 57 kDa
  • ICC/IF image of ab85555 stained HeLa cells. The cells were 100% Methanol fixed (5 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85555, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% Methanol fixed (5 min) HepG2 cells at 5µg/ml, and in 4% PFA fixed (10 min) HeLa, and HepG2 cells at 5µg/ml.
  • ab85555 staining PKM2 in HeLa cells treated with resveratrol (ab120726), by ICC/IF. Decrease in PKM2 expression correlates with increased concentration of resveratrol as described in literature.
    The cells were incubated at 37°C for 48h in media containing different concentrations of ab120726 (resveratrol) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab85555 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

References for Anti-PKM2 antibody (ab85555)

ab85555 has not yet been referenced specifically in any publications.

Product Wall

I can recommend antibody ab85555; http://www.abcam.com/pkm2-antibody-ab85555.html matching your requirements. This antibody is tested in ICC/IF and WB however IP is not yet tested, so in case you are interested in buying and trying this antibody then I...

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