• Product nameAnti-PKM2 antibody [AT4M3]
    See all PKM2 primary antibodies
  • Description
    Mouse monoclonal [AT4M3] to PKM2
  • Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length Human PKM2 (1-531aa) purified from E.coli (NP_002645).

  • Positive control
    • NIH3T3 and Jurkat cell lysates; HeLa cells



Our Abpromise guarantee covers the use of ab100841 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250 - 1/500. Predicted molecular weight: 58 kDa.
ELISA Use at an assay dependent dilution.
ICC/IF 1/250 - 1/500.


  • FunctionGlycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
  • Tissue specificitySpecifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells.
  • PathwayCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
  • Sequence similaritiesBelongs to the pyruvate kinase family.
  • Post-translational
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationCytoplasm. Nucleus. Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic actvity.
  • Information by UniProt
  • Database links
  • Alternative names
    • CTHBP antibody
    • Cytosolic thyroid hormone binding protein antibody
    • Cytosolic thyroid hormone-binding protein antibody
    • KPYM_HUMAN antibody
    • MGC3932 antibody
    • OIP 3 antibody
    • OIP-3 antibody
    • OIP3 antibody
    • OPA interacting protein 3 antibody
    • Opa-interacting protein 3 antibody
    • p58 antibody
    • PK muscle type antibody
    • PK, muscle type antibody
    • PK2 antibody
    • PK3 antibody
    • PKM antibody
    • PKM2 antibody
    • pykm antibody
    • Pyruvate kinase 2/3 antibody
    • Pyruvate kinase 3 antibody
    • Pyruvate kinase isozymes M1/M2 antibody
    • Pyruvate kinase muscle antibody
    • Pyruvate kinase muscle isozyme antibody
    • pyruvate kinase PKM antibody
    • Pyruvate kinase, muscle 2 antibody
    • TCB antibody
    • THBP1 antibody
    • Thyroid hormone binding protein 1 antibody
    • Thyroid hormone binding protein cytosolic antibody
    • Thyroid hormone-binding protein 1 antibody
    • Tumor M2 PK antibody
    • Tumor M2-PK antibody
    see all

Anti-PKM2 antibody [AT4M3] images

  • All lanes : Anti-PKM2 antibody [AT4M3] (ab100841) at 1/500 dilution

    Lane 1 : NIH3T3 cell lysate
    Lane 2 : Jurkat cell lysate

    Lysates/proteins at 35 µg per lane.

    HRP conjugated goat anti-mouse
    Developed using the ECL technique

    Predicted band size : 58 kDa
  • Immunofluorescence analysis of Human HeLa cells stained with ab100841 at 1/500 dilution with Texas Red (Red). Nuclear staining with Hoechst 3342 (Blue).

References for Anti-PKM2 antibody [AT4M3] (ab100841)

This product has been referenced in:
  • Hamabe A  et al. Role of pyruvate kinase M2 in transcriptional regulation leading to epithelial-mesenchymal transition. Proc Natl Acad Sci U S A 111:15526-31 (2014). Read more (PubMed: 25313085) »
  • Busch T  et al. Keratin 8 phosphorylation regulates keratin reorganization and migration of epithelial tumor cells. J Cell Sci 125:2148-59 (2012). Read more (PubMed: 22344252) »

See all 2 Publications for this product

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Based on the information that you have provided, there are 2 protocol variations that I think may help resolve the situation:
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