Recombinant
RabMAb

Anti-PKM2 antibody [EPR10138(B)] (ab150377)

Overview

  • Product name
    Anti-PKM2 antibody [EPR10138(B)]
    See all PKM2 primary antibodies
  • Description
    Rabbit monoclonal [EPR10138(B)] to PKM2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-P, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Pig
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)

  • Positive control
    • Human skeletal muscle, HeLa, Jurkat and A549 cell lysates; HeLa cells
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab150377 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500.
WB 1/1000 - 1/10000. Predicted molecular weight: 58 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
    • Tissue specificity
      Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells.
    • Pathway
      Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
    • Sequence similarities
      Belongs to the pyruvate kinase family.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
      ISGylated.
    • Cellular localization
      Cytoplasm. Nucleus. Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic actvity.
    • Information by UniProt
    • Database links
    • Alternative names
      • CTHBP antibody
      • Cytosolic thyroid hormone binding protein antibody
      • Cytosolic thyroid hormone-binding protein antibody
      • KPYM_HUMAN antibody
      • MGC3932 antibody
      • OIP 3 antibody
      • OIP-3 antibody
      • OIP3 antibody
      • OPA interacting protein 3 antibody
      • Opa-interacting protein 3 antibody
      • p58 antibody
      • PK muscle type antibody
      • PK, muscle type antibody
      • PK2 antibody
      • PK3 antibody
      • PKM antibody
      • PKM2 antibody
      • pykm antibody
      • Pyruvate kinase 2/3 antibody
      • Pyruvate kinase 3 antibody
      • Pyruvate kinase isozymes M1/M2 antibody
      • Pyruvate kinase muscle antibody
      • Pyruvate kinase muscle isozyme antibody
      • pyruvate kinase PKM antibody
      • Pyruvate kinase, muscle 2 antibody
      • TCB antibody
      • THBP1 antibody
      • Thyroid hormone binding protein 1 antibody
      • Thyroid hormone binding protein cytosolic antibody
      • Thyroid hormone-binding protein 1 antibody
      • Tumor M2 PK antibody
      • Tumor M2-PK antibody
      see all

    Images

    • Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) labelling PKM2 with purified ab150377 at 1/500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).

      Control: PBS only

    • All lanes : Anti-PKM2 antibody [EPR10138(B)] (ab150377) at 1/1000 dilution

      Lane 1 : Human skeletal muscle cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : A549 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labeled goat anti-rabbit antibody at 1/2000 dilution

      Predicted band size: 58 kDa

    • Flow cytometric analysis of permeabilized HeLa cells labeling PKM2 with ab150377 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).

    References

    This product has been referenced in:
    • Li L  et al. iNOS-derived nitric oxide promotes glycolysis by inducing pyruvate kinase M2 nuclear translocation in ovarian cancer. Oncotarget 8:33047-33063 (2017). WB . Read more (PubMed: 28380434) »
    • Yu G  et al. PKM2 regulates neural invasion of and predicts poor prognosis for human hilar cholangiocarcinoma. Mol Cancer 14:193 (2015). IHC-P ; Human . Read more (PubMed: 26576639) »

    See all 3 Publications for this product

    Customer reviews and Q&As

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    Yes - 0.5% TritonX100 in PBS
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Username

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Jun 30 2015

    Application
    Western blot
    Loading amount
    80 µg
    Gel Running Conditions
    Reduced Denaturing (12.5% Acrylamide, 2D gel ph 3-10)
    Sample
    Pig Tissue lysate - other (Muscle Sarcoplasm)
    Specification
    Muscle Sarcoplasm
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Dr. Shannon Cruzen

    Verified customer

    Submitted Sep 29 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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