Overview

  • Product name
    Anti-PKM2 antibody [EPR10139]
    See all PKM2 primary antibodies
  • Description
    Rabbit monoclonal [EPR10139] to PKM2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues within Human PKM2 (P14618).

  • Positive control
    • Human fetal muscle tissue lysate; HeLa; A549 and Jurkat cell lysates; Human kidney and cervical carcinoma tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab154816 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 58 kDa.
IHC-P 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Function
      Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
    • Tissue specificity
      Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells.
    • Pathway
      Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
    • Sequence similarities
      Belongs to the pyruvate kinase family.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
      ISGylated.
    • Cellular localization
      Cytoplasm. Nucleus. Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic actvity.
    • Information by UniProt
    • Database links
    • Alternative names
      • CTHBP antibody
      • Cytosolic thyroid hormone binding protein antibody
      • Cytosolic thyroid hormone-binding protein antibody
      • KPYM_HUMAN antibody
      • MGC3932 antibody
      • OIP 3 antibody
      • OIP-3 antibody
      • OIP3 antibody
      • OPA interacting protein 3 antibody
      • Opa-interacting protein 3 antibody
      • p58 antibody
      • PK muscle type antibody
      • PK, muscle type antibody
      • PK2 antibody
      • PK3 antibody
      • PKM antibody
      • PKM2 antibody
      • pykm antibody
      • Pyruvate kinase 2/3 antibody
      • Pyruvate kinase 3 antibody
      • Pyruvate kinase isozymes M1/M2 antibody
      • Pyruvate kinase muscle antibody
      • Pyruvate kinase muscle isozyme antibody
      • pyruvate kinase PKM antibody
      • Pyruvate kinase, muscle 2 antibody
      • TCB antibody
      • THBP1 antibody
      • Thyroid hormone binding protein 1 antibody
      • Thyroid hormone binding protein cytosolic antibody
      • Thyroid hormone-binding protein 1 antibody
      • Tumor M2 PK antibody
      • Tumor M2-PK antibody
      see all

    Images

    • All lanes : Anti-PKM2 antibody [EPR10139] (ab154816) at 1/1000 dilution

      Lane 1 : Human fetal muscle tissue lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : A549 cell lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 58 kDa

    • Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma, labeling PKM2 using ab154816 at 1/100 dilution.
    • Immunohistochemical analysis of paraffin-embedded human kidney, labeling PKM2 using ab154816 at 1/100 dilution.

    References

    This product has been referenced in:
    • Kimura A  et al. FIR haplodeficiency promotes splicing to pyruvate kinase M2 in mice thymic lymphoma tissues revealed by six-plex tandem mass tag quantitative proteomic analysis. Oncotarget 8:67955-67965 (2017). Read more (PubMed: 28978087) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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