Overview

  • Product name
    Anti-PKM2 (phospho Y105) antibody
    See all PKM2 primary antibodies
  • Description
    Rabbit polyclonal to PKM2 (phospho Y105)
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Human
  • Immunogen

    Synthetic peptide within Human PKM2 aa 100-200 (phospho Y105) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: P14618

  • Positive control
    • This antibody gave a positive signal in F9 Mouse embyronic carcinoma cell line.

Properties

Applications

Our Abpromise guarantee covers the use of ab156856 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 58 kDa).
ELISA Use a concentration of 1 µg/ml.

Target

  • Function
    Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
  • Tissue specificity
    Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells.
  • Pathway
    Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
  • Sequence similarities
    Belongs to the pyruvate kinase family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
    ISGylated.
  • Cellular localization
    Cytoplasm. Nucleus. Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic actvity.
  • Information by UniProt
  • Database links
  • Alternative names
    • CTHBP antibody
    • Cytosolic thyroid hormone binding protein antibody
    • Cytosolic thyroid hormone-binding protein antibody
    • KPYM_HUMAN antibody
    • MGC3932 antibody
    • OIP 3 antibody
    • OIP-3 antibody
    • OIP3 antibody
    • OPA interacting protein 3 antibody
    • Opa-interacting protein 3 antibody
    • p58 antibody
    • PK muscle type antibody
    • PK, muscle type antibody
    • PK2 antibody
    • PK3 antibody
    • PKM antibody
    • PKM2 antibody
    • pykm antibody
    • Pyruvate kinase 2/3 antibody
    • Pyruvate kinase 3 antibody
    • Pyruvate kinase isozymes M1/M2 antibody
    • Pyruvate kinase muscle antibody
    • Pyruvate kinase muscle isozyme antibody
    • pyruvate kinase PKM antibody
    • Pyruvate kinase, muscle 2 antibody
    • TCB antibody
    • THBP1 antibody
    • Thyroid hormone binding protein 1 antibody
    • Thyroid hormone binding protein cytosolic antibody
    • Thyroid hormone-binding protein 1 antibody
    • Tumor M2 PK antibody
    • Tumor M2-PK antibody
    see all

Images

  • All lanes : Anti-PKM2 (phospho Y105) antibody (ab156856) at 1 µg/ml

    Lane 1 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate
    Lane 2 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate with Immunising peptide at 1 µg/ml
    Lane 3 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate with Control peptide at 1 µg/ml

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 58 kDa
    Observed band size: 62 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 37 kDa (possible non-specific binding), 41 kDa (possible non-specific binding), 50 kDa (possible non-specific binding)


    Exposure time: 20 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab156856 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406

  • ab156856 was tested using an Indirect ELISA approach. The wells were coated with peptide (1µg/ml at 100µl/well) overnight at 4°C, followed by a 5% BSA blocking step for 1 hour at room temperature. The primary Ab was then added at a dilution range of 1- 0.00025µg/ml (100µl/well) for 1hr at room temperature. A HRP-conjugated anti-rabbit IgG (heavy and light chain) was used as a secondary antibody at 1:20,000 dilution for 1hr at room temperature.

References

This product has been referenced in:
  • Wei Y  et al. Pyruvate kinase type M2 promotes tumour cell exosome release via phosphorylating synaptosome-associated protein 23. Nat Commun 8:14041 (2017). Read more (PubMed: 28067230) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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