Overview

  • Product nameAnti-PKMYT1 antibody [2B4]
    See all PKMYT1 primary antibodies
  • Description
    Mouse monoclonal [2B4] to PKMYT1
  • Tested applicationsSuitable for: WB, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length Human PKMYT1 produced in HEK293T cells (NP_004194).

  • Positive control
    • HEK293T cell lysate transfected with pCMV6-ENTRY PKMYT1; COS7 cells transfected with pCMV6-ENTRY PKMYT1; Hela and Jurkat cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab114022 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 55 kDa.
Flow Cyt 1/100. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100.

Target

  • FunctionActs as a negative regulator of entry into mitosis (G2 to M transition) by phosphorylation of the CDK1 kinase specifically when CDK1 is complexed to cyclins. Mediates phosphorylation of CDK1 predominantly on 'Thr-14'. Also involved in Golgi fragmentation. May be involved in phosphorylation of CDK1 on 'Tyr-15' to a lesser degree, however tyrosine kinase activity is unclear and may be indirect. May be a downstream target of Notch signaling pathway during eye development.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.
    Contains 1 protein kinase domain.
  • DomainThe membrane-association motif is essential for the localization to membrane of Golgi stack. According to some authors, it is a transmembrane domain; the existence of a transmembrane region is however unproven.
  • Post-translational
    modifications
    Autophosphorylated. Phosphorylated by CDC2-CCNB1 complexes on undefined serine and threonine residues. The phosphorylation by CDC2-CCNB1 complexes may inhibit the catalytic activity.
  • Cellular localizationEndoplasmic reticulum membrane. Golgi apparatus membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • cdc2 inhibitory kinase antibody
    • DKFZp547K1610 antibody
    • FLJ20093 antibody
    • Membrane associated tyrosine and threonine specific cdc2 inhibitory kinase antibody
    • Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase antibody
    • MYT1 antibody
    • Myt1 kinase antibody
    • PKMYT 1 antibody
    • Pkmyt1 antibody
    • PMYT1_HUMAN antibody
    • Protein kinase membrane associated tyrosine/threonine 1 antibody
    • Protein kinase Myt1 antibody
    see all

Anti-PKMYT1 antibody [2B4] images

  • All lanes : Anti-PKMYT1 antibody [2B4] (ab114022) at 1/2000 dilution

    Lane 1 : HEK293T cell lysate transfected with the pCMV6-ENTRY control
    Lane 2 : HEK293T cell lysare transfected with the pCMV6-ENTRY PKMYT1


    Predicted band size : 55 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC200368)
  • Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PKMYT1 using ab114022 at 1/100.
  • Flow cytometric analysis of HeLa cells, using ab114022 at 1/100 (Red) compared to a nonspecific negative control antibody (Blue).
  • Flow cytometric analysis of Jurkat cells, using ab114022 at 1/100 (Red) compared to a nonspecific negative control antibody (Blue).

References for Anti-PKMYT1 antibody [2B4] (ab114022)

ab114022 has not yet been referenced specifically in any publications.

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