Overview

  • Product name
  • Description
    Rabbit polyclonal to PKMYT1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, from an internal sequence of Human PKMYT1 (NP_004194.3).

  • Positive control
    • HeLa cell extracts; Human Breast tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab166719 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 55 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Acts as a negative regulator of entry into mitosis (G2 to M transition) by phosphorylation of the CDK1 kinase specifically when CDK1 is complexed to cyclins. Mediates phosphorylation of CDK1 predominantly on 'Thr-14'. Also involved in Golgi fragmentation. May be involved in phosphorylation of CDK1 on 'Tyr-15' to a lesser degree, however tyrosine kinase activity is unclear and may be indirect. May be a downstream target of Notch signaling pathway during eye development.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.
    Contains 1 protein kinase domain.
  • Domain
    The membrane-association motif is essential for the localization to membrane of Golgi stack. According to some authors, it is a transmembrane domain; the existence of a transmembrane region is however unproven.
  • Post-translational
    modifications
    Autophosphorylated. Phosphorylated by CDC2-CCNB1 complexes on undefined serine and threonine residues. The phosphorylation by CDC2-CCNB1 complexes may inhibit the catalytic activity.
  • Cellular localization
    Endoplasmic reticulum membrane. Golgi apparatus membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • cdc2 inhibitory kinase antibody
    • DKFZp547K1610 antibody
    • FLJ20093 antibody
    • Membrane associated tyrosine and threonine specific cdc2 inhibitory kinase antibody
    • Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase antibody
    • MYT1 antibody
    • Myt1 kinase antibody
    • PKMYT 1 antibody
    • Pkmyt1 antibody
    • PMYT1_HUMAN antibody
    • PPP1R126 antibody
    • Protein kinase membrane associated tyrosine/threonine 1 antibody
    • Protein kinase Myt1 antibody
    • Protein phosphatase 1 regulatory subunit 126 antibody
    see all

Images

  • All lanes : Anti-PKMYT1 antibody (ab166719) at 1/500 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HeLa cell lysate with synthesized peptide

    Predicted band size: 55 kDa

  • Immunohistochemical analysis of Formalin-Fixed, Paraffin-Embedded Human Breast tissue labeling PKMYT1 with ab166719 at 5 µg/ml.

References

ab166719 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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