Overview

  • Product nameAnti-PKN antibody [EPR3237]
    See all PKN primary antibodies
  • Description
    Rabbit monoclonal [EPR3237] to PKN
  • Tested applicationsSuitable for: WB, IP, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human PKN.

  • Positive control
    • WB: Jurkat and Human spleen lysates IHC-P: Human brain tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab108976 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 104 kDa).
IP 1/10.
IHC-P 1/100 - 1/250. Antigen retrieval is recommended.
ICC 1/100 - 1/250.
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    • RelevancePKN belongs to the protein kinase C superfamily. This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis.
    • Cellular localizationCytoplasmic
    • Database links
    • Alternative names
      • DBK antibody
      • DPKN antibody
      • PAK 1 antibody
      • PRK 1 antibody
      • PROTEIN KINASE C RELATED KINASE 1 antibody
      • SERINE/THREONINE PROTEIN KINASE N antibody
      see all

    Anti-PKN antibody [EPR3237] images

    • All lanes : Anti-PKN antibody [EPR3237] (ab108976) at 1/1000 dilution

      Lane 1 : Jurkat lysates
      Lane 2 : Human spleen lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 104 kDa
      Observed band size : 120 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical analysis of PKN in paraffin embedded Human brain tissue, using ab108976 at a 1/100 dilution.

    References for Anti-PKN antibody [EPR3237] (ab108976)

    ab108976 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab108976.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"