Recombinant Anti-PKN antibody [EPR3238] (ab108973)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3238] to PKN
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PKN antibody [EPR3238]
See all PKN primary antibodies -
Description
Rabbit monoclonal [EPR3238] to PKN -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- SH-SY-5Y, U937 and Jurkat cell lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR3238 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab108973 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/1000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 104 kDa).
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Notes |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 104 kDa). |
Target
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Relevance
PKN belongs to the protein kinase C superfamily. This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 5585 Human
- Entrez Gene: 320795 Mouse
- Entrez Gene: 29355 Rat
- SwissProt: Q16512 Human
- SwissProt: P70268 Mouse
- SwissProt: Q63433 Rat
- SwissProt: Q6P748 Rat
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Alternative names
- DBK antibody
- DPKN antibody
- PAK 1 antibody
see all
Images
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All lanes : Anti-PKN antibody [EPR3238] (ab108973) at 1/1000 dilution
Lane 1 : SH-SY-5Y cell lysates
Lane 2 : U937 cell lysates
Lane 3 : Jurkat cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 104 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted? -
Intracellular flow cytometric analysis of PKN in permeabilized Jurkat cells, using a 1/10 dilution of ab108973 (red) or a rabbit IgG (negative) (green).
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab108973 has not yet been referenced specifically in any publications.