• Product nameAnti-PKN2 antibody
    See all PKN2 primary antibodies
  • Description
    Rabbit polyclonal to PKN2
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Chicken, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus
  • Immunogen

    Synthetic peptide corresponding to a region between residue 200 and 250 of human PKN2 (NP_006247.1).

  • Positive control
    • Whole cell lysates from HeLa and 293T cells.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab87812 was affinity purified using an epitope specific to PKN2 immobilized on solid support.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab87812 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesIP: Use at 2 - 5 µg/mg of lysate.
    WB: 1/2000 - 1/10,000. Predicted molecular weight: 112 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionExhibits a preference for highly basic protein substrates.
    • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 1 C2 domain.
      Contains 1 protein kinase domain.
      Contains 3 REM (Hr1) repeats.
    • DomainThe C1 domain does not bind the diacylglycerol (DAG).
    • Post-translational
      Activated by limited proteolysis with trypsin.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cardiolipin activated protein kinase Pak2 antibody
      • MGC150606 antibody
      • MGC71074 antibody
      • PAK 2 antibody
      • PAK2 antibody
      • PKN gamma antibody
      • PKN2 antibody
      • PKN2_HUMAN antibody
      • PRK2 antibody
      • PRKCL2 antibody
      • PRO2042 antibody
      • Protein kinase C like 2 antibody
      • Protein kinase C related kinase 2 antibody
      • Protein kinase C-like 2 antibody
      • Protein kinase N2 antibody
      • Protein-kinase C-related kinase 2 antibody
      • Serine/threonine-protein kinase N2 antibody
      see all

    Anti-PKN2 antibody images

    • All lanes : Anti-PKN2 antibody (ab87812) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg

      Developed using the ECL technique

      Predicted band size : 112 kDa
      Observed band size : 117 kDa (why is the actual band size different from the predicted?)

      Exposure time : 10 seconds
    • Detection of PKN2 in immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab87812 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot.
      Detection: Chemiluminescence with an exposure time of 10 seconds.

    References for Anti-PKN2 antibody (ab87812)

    ab87812 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab87812.
    Please use the links above to contact us or submit feedback about this product.