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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
Purity Immunogen affinity purified
Purification notes ab87812 was affinity purified using an epitope specific to PKN2 immobilized on solid support.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes IP: Use at 2 - 5 µg/mg of lysate.
WB: 1/2000 - 1/10,000. Predicted molecular weight: 112 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Function Exhibits a preference for highly basic protein substrates.
Sequence similarities Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 C2 domain. Contains 1 protein kinase domain. Contains 3 REM (Hr1) repeats.
Domain The C1 domain does not bind the diacylglycerol (DAG).
Post-translational modifications Autophosphorylated. Activated by limited proteolysis with trypsin.
Cellular localization Cytoplasm.
Information by UniProt
Cardiolipin activated protein kinase Pak2 antibody
Anti-PKN2 antibody images
Western blot - PKN2 antibody (ab87812)
All lanes : Anti-PKN2 antibody (ab87812) at 0.04 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Developed using the ECL technique Predicted band size : 112 kDa Observed band size : 117 kDa ( why is the actual band size different from the predicted?) Exposure time : 10 seconds
Immunoprecipitation - PKN2 antibody (ab87812)
Detection of PKN2 in immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab87812 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot.
Detection: Chemiluminescence with an exposure time of 10 seconds.
References for Anti-PKN2 antibody (ab87812)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"