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Product name Anti-PKN2 antibody [E352] See all PKN2 primary antibodies
Rabbit monoclonal [E352] to PKN2
Tested applications Suitable for:
WB, IHC-P, ICC, Flow Cyt, IP more details
Mouse, Rat, Human
A synthetic peptide corresponding to residues near N-terminus of human PKN2
293T cell lysate
human colon carcinoma
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb
® technology. RabMAb ® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purity Tissue culture supernatant
Clone number E352
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 112 kDa (predicted molecular weight: 112 kDa).
Use at an assay dependent concentration.
1/250 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Function Exhibits a preference for highly basic protein substrates.
Sequence similarities Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 C2 domain. Contains 1 protein kinase domain. Contains 3 REM (Hr1) repeats.
Domain The C1 domain does not bind the diacylglycerol (DAG).
Post-translational modifications Autophosphorylated. Activated by limited proteolysis with trypsin.
Cellular localization Cytoplasm.
Information by UniProt
Cardiolipin activated protein kinase Pak2 antibody
Anti-PKN2 antibody [E352] images
Western blot - PKN2 antibody [E352] (ab32395)
Anti-PKN2 antibody [E352] (ab32395) at 1/1000 dilution + 10ug 293T cell lysate Predicted band size : 112 kDa Observed band size : 112 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKN2 antibody [E352] (ab32395)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using ab32395 to stain PKN2
References for Anti-PKN2 antibody [E352] (ab32395)
This product has been referenced in:
et al. Characterization of the interaction of Aha1 with components of the Hsp90 chaperone machine and client proteins. Biochim Biophys Acta 1823:1092-101 (2012).
Read more (PubMed: 22504172) »
et al. Delineation of regions of the Yersinia YopM protein required for interaction with the RSK1 and PRK2 host kinases and their requirement for interleukin-10 production and virulence. Infect Immun 78:3529-39 (2010).
Read more (PubMed: 20515922) »
See all 2 Publications
for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"