Overview

  • Product name
    Anti-PKN2 antibody [E352]
    See all PKN2 primary antibodies
  • Description
    Rabbit monoclonal [E352] to PKN2
  • Tested applications
    Suitable for: WB, IHC-P, ICC, Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues near N-terminus of human PKN2

  • Positive control
    • 293T cell lysate human colon carcinoma
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    E352
  • Isotype
    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab32395 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 112 kDa (predicted molecular weight: 112 kDa).
IHC-P Use at an assay dependent concentration.
ICC 1/250 - 1/500.
Flow Cyt 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/50.

Target

  • Function
    Exhibits a preference for highly basic protein substrates.
  • Sequence similarities
    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 1 protein kinase domain.
    Contains 3 REM (Hr1) repeats.
  • Domain
    The C1 domain does not bind the diacylglycerol (DAG).
  • Post-translational
    modifications
    Autophosphorylated.
    Activated by limited proteolysis with trypsin.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cardiolipin activated protein kinase Pak2 antibody
    • MGC150606 antibody
    • MGC71074 antibody
    • PAK 2 antibody
    • PAK2 antibody
    • PKN gamma antibody
    • PKN2 antibody
    • PKN2_HUMAN antibody
    • PRK2 antibody
    • PRKCL2 antibody
    • PRO2042 antibody
    • Protein kinase C like 2 antibody
    • Protein kinase C related kinase 2 antibody
    • Protein kinase C-like 2 antibody
    • Protein kinase N2 antibody
    • Protein-kinase C-related kinase 2 antibody
    • Serine/threonine-protein kinase N2 antibody
    see all

Images

  • Anti-PKN2 antibody [E352] (ab32395) at 1/1000 dilution + 10ug 293T cell lysate

    Predicted band size : 112 kDa
    Observed band size : 112 kDa
  • Immunohistochemical analysis of paraffin-embedded human colon carcinoma using ab32395 to stain PKN2

References

This product has been referenced in:

See all 4 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (THP-1 line)
Loading amount
10000 cells
Specification
THP-1 line
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

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Verified customer

Submitted Feb 11 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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