Anti-PREP1 antibody (ab154587)
Key features and details
- Rabbit polyclonal to PREP1
- Suitable for: WB, ICC/IF, IP
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-PREP1 antibody -
Description
Rabbit polyclonal to PREP1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow -
Immunogen
Recombinant fragment corresponding to Human PREP1 aa 201-436.
Database link: P55347 -
Positive control
- WB: HepG2 nuclear lysate; IP: HeLa cell lysate; ICC/IF: HeLa cell lysate;
-
General notes
Keep as concentrated solution.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154587 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 48 kDa.
|
|
ICC/IF |
1/100 - 1/1000.
|
|
IP |
1/100 - 1/1000.
|
Notes |
---|
WB
1/500 - 1/3000. Predicted molecular weight: 48 kDa. |
ICC/IF
1/100 - 1/1000. |
IP
1/100 - 1/1000. |
Target
-
Tissue specificity
Ubiquitous. Isoform 2 is expressed in all examined tissues except in bone marrow. -
Sequence similarities
Belongs to the TALE/MEIS homeobox family.
Contains 1 homeobox DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 533382 Cow
- Entrez Gene: 5316 Human
- Entrez Gene: 18771 Mouse
- Entrez Gene: 294322 Rat
- Omim: 602100 Human
- SwissProt: Q2HJ84 Cow
- SwissProt: P55347 Human
- SwissProt: O70477 Mouse
see all -
Alternative names
- Homeobox protein PKNOX1 antibody
- Homeobox protein PREP-1 antibody
- Human homeobox containing protein antibody
see all
Images
-
All lanes : Anti-PREP1 antibody (ab154587) at 1/2000 dilution
Lane 1 : HepG2 whole cell lysate
Lane 2 : HepG2 nuclear lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 48 kDa
10% SDS PAGE -
HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. They were stained using ab154587 diluted at 1/1000 (green).
Nuclear staining Hoechst 33342 staining (blue). -
HeLa whole cell lysate/extract
A. 40 μg HeLa whole cell lysate/extract
B. Control with 2 μg of preimmune rabbit IgG
C. Immunoprecipitation of PREP1 protein by 2 μg of ab154587Western Blot carried out by ab154587 at a 1/1000 dilution.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab154587 has not yet been referenced specifically in any publications.