Recombinant fragment: MAGDLSAGFF MEELNTYRQK QGVVLKYQEL PNSGPPHDRR FTFQVIIDGR EFPEGEGRSK KEAKNAAAKL AVEILNKEKK AVSPLLLTTT NSSEGLSMGN , corresponding to amino acids 1-101 of Human PKR
Our Abpromise guarantee covers the use of ab58301 in the following tested applications.
|WB||Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 62 kDa.|
|IHC-P||Use a concentration of 3 µg/ml.|
|ICC/IF||Use a concentration of 10 µg/ml.|
Lanes 1, 3 and 5: PKR knockout HAP1 cell lysate (20 µg)
Lanes 2, 4 and 6: Wild-type HAP1 cell lysate (20 µg)
Lanes 1 and 2: Green signal from target - ab58301 observed at 62 kDa
Lanes 3 and 4: Red signal from loading control - ab181602 observed at 37 kDa
Lanes 5 and 6: Merged (red and green) signal
ab58301 was shown to specifically react with PKR when PKR knockout samples were used. Wild-type and PKR knockout samples were subjected to SDS-PAGE. ab58301 and ab181602 (loading control to GAPDH) were diluted to 1 µg/mL and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.