Overview

  • Product nameAnti-PKR antibody
    See all PKR primary antibodies
  • Description
    Mouse monoclonal to PKR
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: MAGDLSAGFF MEELNTYRQK QGVVLKYQEL PNSGPPHDRR FTFQVIIDGR EFPEGEGRSK KEAKNAAAKL AVEILNKEKK AVSPLLLTTT NSSEGLSMGN , corresponding to amino acids 1-101 of Human PKR

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab58301 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 62 kDa.
IHC-P Use a concentration of 3 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionFollowing activation by double-stranded RNA in the presence of ATP, the kinase becomes autophosphorylated and can catalyze the phosphorylation of the translation initiation factor EIF2S1, which leads to an inhibition of the initiation of protein synthesis. Double-stranded RNA is generated during the course of a viral infection.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
    Contains 2 DRBM (double-stranded RNA-binding) domains.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated on several Ser and Thr residues. Autophosphorylation of Thr-451 is dependent on Thr-446 and is stimulated by dsRNA binding and dimerization. Autophosphorylation apparently leads to the activation of the kinase.
  • Information by UniProt
  • Database links
  • Alternative names
    • Double stranded RNA activated protein kinase; antibody
    • E2AK2_HUMAN antibody
    • eIF-2A protein kinase 2 antibody
    • EIF2AK1 antibody
    • EIF2AK2 antibody
    • Eukaryotic translation initiation factor 2 alpha kinase 2 antibody
    • Eukaryotic translation initiation factor 2-alpha kinase 2 antibody
    • HGNC:9437 antibody
    • Interferon induced double stranded RNA activated protein kinase antibody
    • Interferon inducible elF2 alpha kinase antibody
    • Interferon inducible RNA dependent protein kinase antibody
    • Interferon-induced, double-stranded RNA-activated protein kinase antibody
    • Interferon-inducible RNA-dependent protein kinase antibody
    • MGC126524 antibody
    • P1/eIF-2A protein kinase antibody
    • P1/eIF2A protein kinase antibody
    • p68 kinase antibody
    • PKR antibody
    • PPP1R83 antibody
    • PRKR antibody
    • Protein kinase interferon inducible double stranded RNA dependent antibody
    • Protein kinase RNA activated antibody
    • Protein kinase RNA-activated antibody
    • Protein phosphatase 1 regulatory subunit 83 antibody
    • Serine/threonine protein kinase TIK antibody
    • Tyrosine protein kinase EIF2AK2 antibody
    see all

Anti-PKR antibody images



  • Predicted band size : 62 kDa

    Lanes 1, 3 and 5: PKR knockout HAP1 cell lysate (20 µg)
    Lanes 2, 4 and 6: Wild-type HAP1 cell lysate (20 µg)
    Lanes 1 and 2: Green signal from target - ab58301 observed at 62 kDa
    Lanes 3 and 4: Red signal from loading control - ab181602 observed at 37 kDa
    Lanes 5 and 6: Merged (red and green) signal
    ab58301 was shown to specifically react with PKR when PKR knockout samples were used. Wild-type and PKR knockout samples were subjected to SDS-PAGE. ab58301 and ab181602 (loading control to GAPDH) were diluted to 1 µg/mL and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • PKR antibody (ab58301) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human cerebral cortex.


  • Predicted band size : 62 kDa
    PKR antibody (ab58301) at 1ug/lane + MCF-7 cell lysate at 25ug/lane.
  • ab58301 at 10 ug/ml staining PKR in human Hella cells by Immunocytochemistry/ Immunofluorescence.

References for Anti-PKR antibody (ab58301)

This product has been referenced in:
  • Blalock WL  et al. Multiple forms of PKR present in the nuclei of acute leukemia cells represent an active kinase that is responsive to stress. Leukemia 25:236-45 (2011). WB ; Human . Read more (PubMed: 21072047) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"