Purification notesab79310 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against
non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
1/50 - 1/100.
FunctionFollowing activation by double-stranded RNA in the presence of ATP, the kinase becomes autophosphorylated and can catalyze the phosphorylation of the translation initiation factor EIF2S1, which leads to an inhibition of the initiation of protein synthesis. Double-stranded RNA is generated during the course of a viral infection.
Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily. Contains 2 DRBM (double-stranded RNA-binding) domains. Contains 1 protein kinase domain.
Post-translational modificationsAutophosphorylated on several Ser and Thr residues. Autophosphorylation of Thr-451 is dependent on Thr-446 and is stimulated by dsRNA binding and dimerization. Autophosphorylation apparently leads to the activation of the kinase.
Interferon induced double stranded RNA activated protein kinase antibody
Interferon inducible elF2 alpha kinase antibody
Interferon inducible RNA dependent protein kinase antibody
Interferon-induced, double-stranded RNA-activated protein kinase antibody
Interferon-inducible RNA-dependent protein kinase antibody
P1/eIF-2A protein kinase antibody
P1/eIF2A protein kinase antibody
p68 kinase antibody
Protein kinase interferon inducible double stranded RNA dependent antibody
Protein kinase RNA activated antibody
Protein kinase RNA-activated antibody
Protein phosphatase 1 regulatory subunit 83 antibody
Serine/threonine protein kinase TIK antibody
Tyrosine protein kinase EIF2AK2 antibody
Anti-PKR (phospho T258) antibody images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PKR (phospho T258) antibody (ab79310)
ab79310, at 1/50 dilution, staining PKR in human breast carcinoma tissue by Immunohistochemistry in the presence (right) or absence (left) of immunizing peptide. Formalin-fixed, paraffin-embedded section.
Western blot - PKR (phospho T258) antibody (ab79310)
All lanes : Anti-PKR (phospho T258) antibody (ab79310) at 1/500 dilution
Lane 1 : Jurkat cells starved for 24 hours Lane 2 : Jurkat cells starved for 24 hours with Immunizing phosphopeptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 62 kDa Observed band size : 62 kDa
References for Anti-PKR (phospho T258) antibody (ab79310)
has not yet been referenced specifically in any publications.
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