PL6 overexpression 293T lysate (whole cell) (ab94331)
Overview
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Product name
PL6 overexpression 293T lysate (whole cell) -
General notes
ab94331 is a 293T cell transfected lysate in which Human PL6 has been transiently over-expressed using a pCMV-PL6 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
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Tested applications
Suitable for: WBmore details
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.80
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol -
Concentration information loading...
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Research areas
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Background
PL6 is an integral membrane protein with signal transduction activity.
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab94331 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent dilution.
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Notes |
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WB
Use at an assay dependent dilution. |
Images
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ab94331 at 15µg/lane on an SDS-PAGE gel.
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All lanes : Anti-PL6 antibody (ab56868) at 1/500 dilution
Lane 1 :PL6 overexpression 293T lysate (whole cell) (ab94331)
Lane 2 : 293T Non Transfected Lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab94331 has not yet been referenced specifically in any publications.