Overview

  • Product nameAnti-PLA2G12B antibody
    See all PLA2G12B primary antibodies
  • Description
    Rabbit polyclonal to PLA2G12B
  • Tested applicationsICC/IF, WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A 15 amino acid peptide from near the N terminal residues of human PLA2G12B (NP_115951)

  • Positive control
    • Mouse liver lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab85069 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/200 - 1/500. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
ELISA Use at an assay dependent dilution.

Target

  • RelevancePhospholipase A2 (EC 3.1.1.4) catalyzes the release of fatty acids from glycero-3-phosphocholines. The best known varieties are the digestive enzymes secreted as zymogens by the pancreas of mammals. Sequences of pancreatic PLA2 enzymes from a variety of mammals have been reported. One striking feature of these enzymes is their close homology to venom phospholipases of snakes. Other forms of PLA2 have been isolated from brain, liver, lung, spleen, intestine, macrophages, leukocytes, erythrocytes, inflammatory exudates, chondrocytes, and platelets (Seilhamer et al., 1986 [PubMed 3028739]).
  • Cellular localizationSecreted
  • Database links
  • Alternative names
    • 2010002E04Rik antibody
    • FKSG71 antibody
    • Group XIIB phospholipase A2 antibody
    • Group XIII secreted phospholipase A2 antibody
    • GXIIB antibody
    • GXIIIsPLA2 antibody
    • MGC138151 antibody
    • Phospholipase A2, group XIIB antibody
    • PLA2G12B antibody
    • PLA2G13 antibody
    see all

Anti-PLA2G12B antibody images

  • Anti-PLA2G12B antibody (ab85069) at 1/250 dilution + mouse liver lysates

    Predicted band size : 22 kDa
    Observed band size : 22 kDa
  • ICC/IF image of ab85069 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85069, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-PLA2G12B antibody (ab85069)

This product has been referenced in:
  • Li X  et al. Hepatocyte nuclear factor 4a and downstream secreted phospholipase A2 GXIIB regulate production of infectious hepatitis C virus. J Virol 88:612-27 (2014). Read more (PubMed: 24173221) »

See 1 Publication for this product

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Thank you for contacting us. The liver lysate was not prepared in any special way. It was a standard lysis of fresh mouse liver in RIPA buffer with protease inhibitors. Please do not hesitate to contact us if you need any more advice or information.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"