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Synthetic peptide corresponding to Human PLA2G12B (N terminal). A 15 amino acid peptide from near the N terminal residues of human PLA2G12B.
Database link: NP_115951
Our Abpromise guarantee covers the use of ab85069 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|IHC||Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
|WB||1/200 - 1/500. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).|
|IHC-P||1/100 - 1/500.|
ICC/IF image of ab85069 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85069, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunohistochemistry (Formalin/PFa-fixed paraffin-embedded sections) analysis of human fetal liver tissue labelling PLA2G12B with ab85069 at a dilution of 1/100.
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