Overview

  • Product name
  • Description
    Rabbit polyclonal to PLA2G4F
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant protein fragment containing a sequence corresponding to a region within amino acids 379 and 602 (Q68DD2) of PLA2G4F

  • Positive control
    • 293T, A431 whole cell lysate, Raji whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab111551 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with 10 mM citrate buffer pH 6 or tris-EDTA buffer pH 8 before commencing with IHC staining protocol.
WB 1/500 - 1/3000. Predicted molecular weight: 71 kDa.

Target

  • Function
    Calcium-dependent phospholipase A2 that selectively hydrolyzes glycerophospholipids in the sn-2 position. Has higher enzyme activity for phosphatidylethanolamine than phosphatidylcholine.
  • Sequence similarities
    Contains 1 C2 domain.
    Contains 1 PLA2c domain.
  • Domain
    The N-terminal C2 domain associates with lipid membranes upon calcium binding. It modulates enzyme activity by presenting the active site to its substrate in response to elevations of cytosolic Ca(2+).
  • Cellular localization
    Cytoplasm > cytosol. Lysosome membrane. Translocates to lysosomal membranes in a calcium-dependent fashion.
  • Information by UniProt
  • Database links
  • Alternative names
    • cPLA2-zeta antibody
    • Cytosolic phospholipase A2 zeta antibody
    • DKFZp666G192 antibody
    • DKFZp781B229 antibody
    • PA24F_HUMAN antibody
    • Phospholipase A2 group IVF antibody
    • Pla2g4f antibody
    • PLA2G4FZ antibody
    see all

Images

  • Anti-PLA2G4F antibody (ab111551) at 1/1000 dilution + Raji whole cell lysate at 30 µg

    Predicted band size : 71 kDa
  • ab111551 at 1/500 dilution, staining PLA2G4F by IHC-P (gastric ca).

References

ab111551 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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