Anti-Placental alkaline phosphatase (PLAP) antibody [8B6] (ab33)


  • Product nameAnti-Placental alkaline phosphatase (PLAP) antibody [8B6]
    See all Placental alkaline phosphatase (PLAP) primary antibodies
  • Description
    Mouse monoclonal [8B6] to Placental alkaline phosphatase (PLAP)
  • SpecificityHuman placental alkaline phosphatase, Regan and Nagao isoenzymes. No cross reaction with other isoenzymes of AP.
  • Tested applicationsSuitable for: ELISA, IHC-P, RIA, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    HEp-2 cells with boosted surface expression of PLAP

  • Positive control
    • Placenta.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: None
    Constituents: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityMonoclonal
  • Clone number8B6
  • MyelomaP3-NS1/1-Ag4-1
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas


Our Abpromise guarantee covers the use of ab33 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IHC-P 1/250 - 1/500.
RIA Use at an assay dependent concentration.
IHC-Fr 1/250 - 1/500.


  • Sequence similaritiesBelongs to the alkaline phosphatase family.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alkaline phosphatase antibody
    • Alkaline phosphatase placental antibody
    • Alkaline phosphatase placental type antibody
    • Alkaline phosphatase Regan isozyme antibody
    • ALP antibody
    • Alp1 antibody
    • ALPP antibody
    • FLJ61142 antibody
    • Germ-cell alkaline phosphatase antibody
    • nagao isozyme antibody
    • OTTHUMP00000164354 antibody
    • PALP antibody
    • Placental alkaline phosphatase 1 antibody
    • placental heat-stable alkaline phosphatase antibody
    • placental type antibody
    • PLAP antibody
    • PLAP-1 antibody
    • PLAP1 antibody
    • PPB1_HUMAN antibody
    see all

References for Anti-Placental alkaline phosphatase (PLAP) antibody [8B6] (ab33)

This product has been referenced in:
  • Sharp TM  et al. Inhibition of cellular protein secretion by norwalk virus nonstructural protein p22 requires a mimic of an endoplasmic reticulum export signal. PLoS One 5:e13130 (2010). ICC/IF ; Human . Read more (PubMed: 20976190) »
  • Zhang L  et al. Alteration in the IL-2 signal peptide affects secretion of proteins in vitro and in vivo. J Gene Med 7:354-65 (2005). ELISA ; Human . Read more (PubMed: 15619290) »

See all 4 Publications for this product

Product Wall

Thank you for your enquiry. Unfortunately we have not tested ab33 against mouse so we do not know whether it is suitable. The antibody was raised against the whole protein, and although the mouse and human protein show 75% homology we do not know wh...

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Thank you for your enquiry. According to the database SwissProt the MW of the precursor PLAP is 57kDa and it is glycosylated therefore its weight should be higher than this. A paper by Webb et al (Placenta. 1986 Sep-Oct;7(5):405-15.Isolation of pl...

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I'm afraid that we do not have any data regarding the suitablity of this antibody for WB.

This antibody has not been tested in flow cytometry.

This antibody has not been tested in immunofluorescence.

This monoclonal was raised against the whole protein, and in the absence of any epitope mapping studies, I am afraid that we do not know against which part of the protein the antibody is reactive.

Vlad We do not have any data on this antibody's inhibiting activity in vivo or in vitro. We are about to implement a trial 'try risk free' scheme which says that if an antibody is purchased and used in an experiment outside that written on the datashe...

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This antibody has not yet been tested in Western blotting.