Anti-Placental alkaline phosphatase (PLAP) antibody [HD 11F7] (ab20228)
Key features and details
- Mouse monoclonal [HD 11F7] to Placental alkaline phosphatase (PLAP)
- Suitable for: RIA, ELISA, IHC
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-Placental alkaline phosphatase (PLAP) antibody [HD 11F7]
See all Placental alkaline phosphatase (PLAP) primary antibodies -
Description
Mouse monoclonal [HD 11F7] to Placental alkaline phosphatase (PLAP) -
Host species
Mouse -
Tested applications
Suitable for: RIA, ELISA, IHCmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human Placental alkaline phosphatase (PLAP). Fresh Hep-2 cells followed by booster injections of fresh Hep-2 cells that had their surface expression of PLAP boosted in culture.
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General notes
HD 11F7 has potential uses in the diagnosis of ovarian and testicular tumours. The antibody detects both Regan and Nagao isoenzymes.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading...
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Purity
Protein A/G purified -
Primary antibody notes
HD 11F7 has potential uses in the diagnosis of ovarian and testicular tumours. The antibody detects both Regan and Nagao isoenzymes. -
Clonality
Monoclonal -
Clone number
HD 11F7 -
Myeloma
P3-NS1/1-Ag4-1 -
Isotype
IgG2b -
Light chain type
unknown -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab20228 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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RIA |
Use at an assay dependent concentration.
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ELISA |
Use at an assay dependent concentration.
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IHC |
Use at an assay dependent concentration.
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Notes |
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RIA
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. |
IHC
Use at an assay dependent concentration. |
Target
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Sequence similarities
Belongs to the alkaline phosphatase family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 250 Human
- Omim: 171800 Human
- SwissProt: P05187 Human
- Unigene: 284255 Human
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Alternative names
- Alkaline phosphatase antibody
- Alkaline phosphatase placental antibody
- Alkaline phosphatase placental type antibody
see all
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab20228 has been referenced in 2 publications.
- Durbin H et al. Monoclonal antibodies to placental alkaline phosphatase: preclinical evaluation in a human xenograft tumour model of F(ab')2 and Fab fragments. Int J Cancer Suppl 2:59-66 (1988). PubMed: 3162446
- Durbin H et al. Production of monoclonal antibodies to placental alkaline phosphatase: preliminary characterisation includes identification of one antibody reactive with routinely fixed histological preparations. Int J Cancer Suppl 2:50-8 (1988). PubMed: 2450848