Overview

  • Product name
    Anti-Placental lactogen antibody
    See all Placental lactogen primary antibodies
  • Description
    Rabbit polyclonal to Placental lactogen
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Purified human placental lactogen

  • Positive control
    • Placenta

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.40
    Preservative: 0.1% Sodium azide
    Constituents: 0.0268% PBS, BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab15554 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 25 kDa.
IHC-P 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function
    Produced only during pregnancy and is involved in stimulating lactation, fetal growth and metabolism. Does not interact with GHR but only activates PRLR through zinc-induced dimerization.
  • Sequence similarities
    Belongs to the somatotropin/prolactin family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Choriomammotropin antibody
    • Chorionic somatomammotropin A antibody
    • chorionic somatomammotropin B antibody
    • chorionic somatomammotropin hormone 1 (placental lactogen) antibody
    • Chorionic somatomammotropin hormone 1 antibody
    • chorionic somatomammotropin hormone 2 antibody
    • Chorionic somatomammotropin hormone antibody
    • CS-2 antibody
    • CS1 antibody
    • CSA antibody
    • CSB antibody
    • CSH_HUMAN antibody
    • CSH1 antibody
    • CSH2 antibody
    • CSMT antibody
    • hCS-B antibody
    • hCSA antibody
    • Lactogen antibody
    • PL antibody
    • PL1 antibody
    • Placental lactogen antibody
    see all

Anti-Placental lactogen antibody images

  • ab15554 staining Human placenta by Immunohistochemistry (FFPE-sections).

  • Anti-Placental lactogen antibody (ab15554) at 1/100 dilution + Human recombinant hPL at 0.1 µg

    Secondary
    HRP conjugated donkey anti-rabbit IgG
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 25 kDa
    Observed band size : 25 kDa
    Additional bands at : 10 kDa,14 kDa,45 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 5 minutes

    This image is courtesy of an Abreview submitted by Dr Stoil Dmitrov

    See Abreview

  • ab15554 at 1/200 staining human BeWo (placenta choriocarcinoma) cells by ICC/IF. The cells were paraformaldehyde fixed and blocked with BSA prior to incubation with the antibody for 30 minutes. A rhodamine conjugated goat anti-rabbit antibody was used as the secondary. The left hand panel of the image shows DAPI counterstain.

    See Abreview

  • Anti-Placental lactogen antibody (ab15554) at 1/25 dilution + Human placenta lysate

    Predicted band size : 25 kDa
    Observed band size : 23 kDa (why is the actual band size different from the predicted?)

References for Anti-Placental lactogen antibody (ab15554)

This product has been referenced in:
  • Drukker M  et al. Isolation of primitive endoderm, mesoderm, vascular endothelial and trophoblast progenitors from human pluripotent stem cells. Nat Biotechnol 30:531-42 (2012). ICC/IF ; Human . Read more (PubMed: 22634564) »
  • Adachi K  et al. Role of SOX2 in maintaining pluripotency of human embryonic stem cells. Genes Cells : (2010). Read more (PubMed: 20384793) »

See all 3 Publications for this product

Product Wall

Unfortunately the PI of the antibody is not known.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (BeWo, placenta, choriocarcinoma)
Specification
BeWo, placenta, choriocarcinoma
Fixative
Paraformaldehyde
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1%
Username

Dr. Stoil Dmitrov

Verified customer

Submitted Apr 11 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Recombinant protein (Human recombinant hPL)
Loading amount
0.1 µg
Specification
Human recombinant hPL
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5%
Username

Dr. Stoil Dmitrov

Verified customer

Submitted Apr 11 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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