Anti-Placental Protein 14 / Glycodelin A antibody (ab123318)

Overview

  • Product nameAnti-Placental Protein 14 / Glycodelin A antibody
    See all Placental Protein 14 / Glycodelin A primary antibodies
  • Description
    Rabbit polyclonal to Placental Protein 14 / Glycodelin A
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 72-101 of Human Placental Protein 14/ Glycodelin A (NP_001018059.1, NP_002562.2).

  • Positive control
    • WiDr cell line lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage bufferPreservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified through a protein A column, followed by peptide affinity purification.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab123318 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 18 kDa.

Target

  • FunctionThis protein is, quantitatively, the main protein synthesized and secreted in the endometrium from mid-luteal phase of the menstrual cycle and during the first semester of pregnancy.
  • Sequence similaritiesBelongs to the calycin superfamily. Lipocalin family.
  • Post-translational
    modifications
    At least two differentially glycosylated forms are found. Glycodelin-A (GdA) (amniotic fluid) with contraceptive and immunosuppressive activities and glycodelin-S (Gds) (seminal plasma) whose role is not yet known. The gender-specific glycosylation may serve to regulate key process involved in human reproduction.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha uterine protein antibody
    • gD antibody
    • GdA antibody
    • GdF antibody
    • GdS antibody
    • Glycodelin A antibody
    • Glycodelin antibody
    • Glycodelin F antibody
    • Glycodelin S antibody
    • MGC138509 antibody
    • MGC142288 antibody
    • PAEG antibody
    • PAEP antibody
    • PAEP_HUMAN antibody
    • PEG antibody
    • PEP antibody
    • Placental protein 14 antibody
    • PP14 antibody
    • Pregnancy-associated endometrial alpha-2 globulin antibody
    • Progestagen-associated endometrial protein antibody
    • Progesterone-associated endometrial protein antibody
    see all

Anti-Placental Protein 14 / Glycodelin A antibody images

  • Anti-Placental Protein 14 / Glycodelin A antibody (ab123318) at 1/100 dilution + WiDr cell line lysates at 35 µg
    Developed using the ECL technique

    Predicted band size : 18 kDa

References for Anti-Placental Protein 14 / Glycodelin A antibody (ab123318)

ab123318 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (OV90)
Permeabilization Yes - 0.25% Triton-X 100 in PBS
Specification OV90
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Fixative Formaldehyde
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Submitted Oct 06 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (MDA231 human tumor from Balb/C nude mice)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH6
Permeabilization No
Specification MDA231 human tumor from Balb/C nude mice
Blocking step innovex background buster as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 20°C
Fixative Formaldehyde
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Submitted Oct 05 2016

Application Western blot
Sample Human Cell lysate - whole cell (OV90 cells)
Gel Running Conditions Reduced Denaturing (4-20% Tris Glycine)
Loading amount 50000 cells
Specification OV90 cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Submitted Aug 31 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"