Recombinant
RabMAb

Anti-PLAGL1 / ZAC antibody [EPR7523] (ab129063)

Overview

  • Product name
    Anti-PLAGL1 / ZAC antibody [EPR7523]
    See all PLAGL1 / ZAC primary antibodies
  • Description
    Rabbit monoclonal [EPR7523] to PLAGL1 / ZAC
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues on the C-terminus of Human PLAGL1/ ZAC.

  • Positive control
    • Human fetal liver and Human fetal kidney, 293T, HepG2 and SH-SY5Y lysates. Permeabilized HepG2 cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab129063 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Function
      Shows weak transcriptional activatory activity. Transcriptional regulator of the type 1 receptor for pituitary adenylate cyclase-activating polypeptide.
    • Sequence similarities
      Belongs to the krueppel C2H2-type zinc-finger protein family.
      Contains 7 C2H2-type zinc fingers.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • DKFZp781P1017 antibody
      • Lost on transformation 1 antibody
      • Lost on Transformation antibody
      • LOT-1 antibody
      • LOT1 antibody
      • MGC126275 antibody
      • MGC126276 antibody
      • PLAG like 1 antibody
      • PLAGL1 antibody
      • PLAL1_HUMAN antibody
      • Pleiomorphic adenoma gene like 1 antibody
      • Pleiomorphic adenoma gene like protein 1 antibody
      • Pleiomorphic adenoma-like protein 1 antibody
      • Tumor supressor ZAC antibody
      • ZAC antibody
      • ZAC tumor supressor antibody
      • ZAC1 antibody
      • Zinc finger protein PLAGL1 antibody
      see all

    Images

    • All lanes : Anti-PLAGL1 / ZAC antibody [EPR7523] (ab129063) at 1/10000 dilution

      Lane 1 : Human fetal liver lysate
      Lane 2 : Human fetal kidney lysate
      Lane 3 : 293T cell lysate
      Lane 4 : HepG2 cell lysate
      Lane 5 : SH-SY5Y cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 51 kDa
      Observed band size : 51 kDa
    • ab129063 at 1/10 dilution staining PLAGL1 / ZAC in permeabilized HepG2 cells by Flow Cytometry (red) or a rabbit IgG negative (green).
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:
    • Godlewski J  et al. PLAGL1 (ZAC1/LOT1) Expression in Clear Cell Renal Cell Carcinoma: Correlations with Disease Progression and Unfavorable Prognosis. Anticancer Res 36:617-24 (2016). Read more (PubMed: 26851016) »
    • Iglesias-Platas I  et al. Altered expression of the imprinted transcription factor PLAGL1 deregulates a network of genes in the human IUGR placenta. Hum Mol Genet N/A:N/A (2014). Read more (PubMed: 24993786) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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