Overview

  • Product nameAnti-PLAGL2 antibody
    See all PLAGL2 primary antibodies
  • Description
    Rabbit polyclonal to PLAGL2
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to amino acids 236-344 of Human PLAGL2

  • Positive control
    • Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells); Human lymph node tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab121239 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

WB 1/250 - 1/500.
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. pH 6.0

Target

  • FunctionShows weak transcriptional activatory activity.
  • Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 6 C2H2-type zinc fingers.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • C2H2 type zinc finger protein antibody
    • KIAA0198 antibody
    • PLAGL2 antibody
    • PLAL2_HUMAN antibody
    • Pleiomorphic adenoma gene-like 2 antibody
    • Pleiomorphic adenoma-like protein 2 antibody
    • Zinc finger protein PLAGL2 antibody
    • ZNF900 antibody
    see all

Anti-PLAGL2 antibody images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121239 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • All lanes : Anti-PLAGL2 antibody (ab121239) at 1/250 dilution

    Lane 1 : Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
    Lane 2 : Negative control (vector only transfected HEK293T lysate)
    Lane 3 : Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)
  • Immunohistochemical staining of Paraffin embedded Human lymph node tissue, using 1/35 ab121239, shows strong cytoplasmic positivity in reaction center cells.

References for Anti-PLAGL2 antibody (ab121239)

This product has been referenced in:
  • Guo J  et al. Overexpression of Pleomorphic Adenoma Gene-Like 2 Is a Novel Poor Prognostic Marker of Prostate Cancer. PLoS One 11:e0158667 (2016). Read more (PubMed: 27537362) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"