Anti-Plakophilin 2/PKP2 antibody (ab223757)
Key features and details
- Rabbit polyclonal to Plakophilin 2/PKP2
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Plakophilin 2/PKP2 antibody
See all Plakophilin 2/PKP2 primary antibodies -
Description
Rabbit polyclonal to Plakophilin 2/PKP2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human Plakophilin 2/PKP2 aa 50-200.
Database link: Q99959 -
Positive control
- WB: Caco-2 cell lysate. IHC-P: Human heart muscle tissue. ICC/IF: A431 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab223757 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 97.4 kDa.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 97.4 kDa. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
May play a role in junctional plaques. -
Tissue specificity
Detected in heart right ventricle (at protein level). Widely expressed. Found at desmosomal plaques in simple and stratified epithelia and in non-epithelial tissues such as myocardium and lymph node follicles. In most stratified epithelia found in the desmosomes of the basal cell layer and seems to be absent from suprabasal strata. -
Involvement in disease
Familial arrhythmogenic right ventricular dysplasia 9 -
Sequence similarities
Belongs to the beta-catenin family.
Contains 8 ARM repeats. -
Cellular localization
Nucleus. Cell junction > desmosome. Nuclear and associated with desmosomes. - Information by UniProt
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Database links
- Entrez Gene: 5318 Human
- Omim: 602861 Human
- SwissProt: Q99959 Human
- Unigene: 164384 Human
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Alternative names
- ARVD 9 antibody
- ARVD9 antibody
- PKP 2 antibody
see all
Images
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All lanes : Anti-Plakophilin 2/PKP2 antibody (ab223757) at 0.4 µg/ml
Lane 1 : Human cell line Caco-2
Lane 2 : Human cell line SK-MEL-30
Predicted band size: 97.4 kDa -
Immunohistochemical analyssi of paraffin embedded human heart muscle tissue staining PKP2 with ab223757 at 1/50 dilution.
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PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for Plakophilin 2/PKP2 (green) using ab223757 at 4 µg/ml in ICC/IF.
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Immunohistochemical analyssi of paraffin embedded human skeletal muscle tissue staining PKP2 with ab223757 at 1/50 dilution.
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Immunohistochemical analyssi of paraffin embedded human liver tissue staining PKP2 with ab223757 at 1/50 dilution.
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Immunohistochemical analyssi of paraffin embedded human testis tissue staining PKP2 with ab223757 at 1/50 dilution.
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Paraffin embedded sections of human heart muscle tissue stained for Plakophilin 2/PKP2 with ab223757 at 1/50 dilution in immunohistochemical analysis.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab223757 has not yet been referenced specifically in any publications.