The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesICC: Use at an assay dependent dilution.
Note: cells need to be methanol fixed.
IHC-Fr: 1/25 - 1/200, using avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent.
Note: For optimal results, fixed cells or tissues should be treated with 0.2% Triton-X 100 for 5 min. prior to incubation with primary antibody.
IP: Use at an assay dependent dilution.
WB: 1/100 - 1/1000. Predicted molecular weight: 87 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionMay play a role in junctional plaques.
Tissue specificityFound in desmosomes of most simple and stratified epithelia. Not found in foreskin fibroblasts and various sarcoma-derived cell lines. Beside dendritic reticular cells of lymphatic follicles not found in non-epithelial desmosome-bearing tissues.
Sequence similaritiesBelongs to the beta-catenin family. Contains 8 ARM repeats.
Cellular localizationNucleus. Cell junction > desmosome. Nuclear and associated with desmosomes.