Recombinant
RabMAb

Anti-Plakophilin 3 antibody [EPR5560] (ab109441)

Overview

  • Product name
    Anti-Plakophilin 3 antibody [EPR5560]
    See all Plakophilin 3 primary antibodies
  • Description
    Rabbit monoclonal [EPR5560] to Plakophilin 3
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human Plakophilin 3.

  • Positive control
    • Caco2 and HACAT cell lysates; Human breast and kidney tissues.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab109441 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 87 kDa (predicted molecular weight: 87 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    Anti-Plakophilin 3 antibody [EPR5560] images

    • ab109441 at 1/100 dilution staining Human paraffin-embedded breast tissue.
    • ab109441 at 1/100 dilution staining paraffin-embedded Human kidney tissue.
    • All lanes : Anti-Plakophilin 3 antibody [EPR5560] (ab109441) at 1/10000 dilution

      Lane 1 : Caco2 cell lysate
      Lane 2 : HACAT cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 87 kDa
      Observed band size : 87 kDa
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-Plakophilin 3 antibody [EPR5560] (ab109441)

    This product has been referenced in:
    • Zhou L  et al. Mice with Hepatic Loss of the Desmosomal Protein ?-Catenin Are Prone to Cholestatic Injury and Chemical Carcinogenesis. Am J Pathol 185:3274-89 (2015). Read more (PubMed: 26485505) »

    See 1 Publication for this product

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    We would not recommend to freeze the antibody again once it has been thawed and used on a few samples. These repeated freeze/thaw cycles could damage the antibody. Instead, we would suggest to prepare and freeze s...

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    Thank you for your enquiry.

    I can confirm that ab109441 has been tested successfully in IHC-P at a dilution of 1/100 - 1/250, as stated on the datasheet. Please note this is a guideline only, and may require some further optimization.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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