Anti-Plasma Kallikrein 1B antibody [13G11] (ab1006)

Overview

  • Product nameAnti-Plasma Kallikrein 1B antibody [13G11]
    See all Plasma Kallikrein 1B primary antibodies
  • Description
    Mouse monoclonal [13G11] to Plasma Kallikrein 1B
  • SpecificityThis antibody recognizes two variants (88 kd and 85 kd) of prekallikrein and kallikrein.
  • Tested applicationsSuitable for: IHC-P, ELISA, Inhibition Assay, WBmore details
  • Species reactivity
    Reacts with: Rat, Human, Chimpanzee, Baboon, Rhesus monkey
  • Immunogen

    Full length native prekallikrein protein (purified) (Human)

  • EpitopeThe specific epitope, found on the prekallikrein / kallikrein heavy chain is involved in the interaction between prekallikrein and factor XIIa.

Properties

Applications

Our Abpromise guarantee covers the use of ab1006 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ELISA Use at an assay dependent concentration.
Inhibition Assay Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
Dilute in PBS or medium which is identical to that used in the assay system. This antibody inhibits activation of prekallikrein by approximately 60-80%.

Target

  • FunctionThe enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.
  • Involvement in diseaseDefects in KLKB1 are the cause of prekallikrein deficiency (PKK deficiency) [MIM:612423]; also known as Fletcher factor deficiency. This disorder is a blood coagulation defect.
  • Sequence similaritiesBelongs to the peptidase S1 family. Plasma kallikrein subfamily.
    Contains 4 apple domains.
    Contains 1 peptidase S1 domain.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Fletcher factor antibody
    • kallikrein B plasma antibody
    • Kallikrein B plasma (Fletcher factor) 1 antibody
    • Kallikrein B, plasma 1 antibody
    • Kininogenin antibody
    • KLK3 antibody
    • KLKB1 antibody
    • KLKB1_HUMAN antibody
    • Plasma kallikrein antibody
    • Plasma kallikrein B1 antibody
    • Plasma kallikrein light chain antibody
    • Plasma prekallikrein antibody
    • PPK antibody
    • Prekallikrein antibody
    see all

Anti-Plasma Kallikrein 1B antibody [13G11] images


  • Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 2 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

  • ab1006 (4µg/ml) staining plasma kallikrein in human liver, using an automated system (DAKO Autostainer Plus). Using this protocol there is weak cytoplasmic staining of a subpopulation of hepatocytes.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

References for Anti-Plasma Kallikrein 1B antibody [13G11] (ab1006)

This product has been referenced in:
  • Liu J  et al. Hyperglycemia-induced cerebral hematoma expansion is mediated by plasma kallikrein. Nat Med 17:206-10 (2011). Functional Studies ; Rat . Read more (PubMed: 21258336) »
  • Morris KE  et al. Autonomic nervous system regulates secretion of anti-inflammatory prohormone SMR1 from rat salivary glands. Am J Physiol Cell Physiol 296:C514-24 (2009). WB ; Rat . Read more (PubMed: 19109528) »

See all 8 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Purified protein (human plasma)
Loading amount 3 µg
Specification human plasma
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Abcam user community

Verified customer

Submitted Dec 31 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (Retina)
Loading amount 30 µg
Specification Retina
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
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Abcam user community

Verified customer

Submitted Dec 31 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - other (Human Vitreous)
Specification Human Vitreous
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

Submitted Feb 12 2007

Unfortunately, we do not have more information about this antibody. We will update the on-line datasheet of this product as soon as we get more data. We apologize for any inconvenience caused.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"