![Western blot - Plasma Kallikrein 1B antibody [13G11] (ab1006) image](#ab1006_1.jpg)
 image](#Plasma-Kallikrein-1B-Primary-antibodies-ab1006-3.jpg)
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Products:Cardiovascular >> Blood >> Serum Proteins
Overview
Product name
Anti-Plasma Kallikrein 1B antibody [13G11]
See all Plasma Kallikrein 1B products (7) ...
Description
Mouse monoclonal [13G11] to Plasma Kallikrein 1B
Specificity
This antibody recognizes two variants (88 kd and 85 kd) of prekallikrein and kallikrein.
Tested applications
IHC-P, ELISA, Inhibition Assay, WBmore details
Cross reactivity
Reacts with
Rat, Human, Chimpanzee, Baboon, Rhesus monkey
Immunogen
Full length native prekallikrein protein (purified) (Human)
Epitope
The specific epitope, found on the prekallikrein / kallikrein heavy chain is involved in the interaction between prekallikrein and factor XIIa.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS, pH7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Clone number
13G11
Isotype
IgG1
Research areas
Cardiovascular >> Blood >> Blood Pressure regulation
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> Kallikreins
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> Kallikreins
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> Kallikreins
Cardiovascular >> Blood >> Coagulation >> Intrinsic
Cardiovascular >> Blood >> Serum Proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab1006 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 4 µg/mlPerform heat mediated antigen retrieval before commencing with IHC staining protocol.
ELISA: Use at an assay dependent dilution.
Inhib: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. (Dilute in PBS or medium which is identical to that used in the assay system. This antibody inhibits activation of prekallikrein by approximately 60-80%.)
Target
Function
The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.
Involvement in disease
Defects in KLKB1 are the cause of prekallikrein deficiency (PKK deficiency) [MIM:612423]; also known as Fletcher factor deficiency. This disorder is a blood coagulation defect.
Sequence similarities
Belongs to the peptidase S1 family. Plasma kallikrein subfamily.
Contains 4 apple domains.
Contains 1 peptidase S1 domain.
Cellular localization
Secreted.
Target information above from: UniProt accessionP03952
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Fletcher factor antibody
- Glandular kallikrein antibody
- kallikrein 1 related peptidase b1 antibody
see all
Database links
- Entrez Gene: 3818 Human
- Entrez Gene: 25048 Rat
- Omim: 229000 Human
- SwissProt: P03952 Human
- SwissProt: P14272 Rat
- Unigene: 237642 Human
- Unigene: 9880 Rat
Anti-Plasma Kallikrein 1B antibody [13G11] images:
Western blot - Plasma Kallikrein 1B antibody [13G11] (ab1006)
![Western blot - Plasma Kallikrein 1B antibody [13G11] (ab1006)](/ps/datasheet/Images/1/ab1006/ab1006_1.jpg)
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 2 minutes
ab1006 staining Plasma Kallikrein 1B by Western Blot.
Lanes 1-6: Human Vitreous (4ug/lane)
Lane 7: PK
Lane 8: PK + Factor XII + HK
An HRP conjugated Goat anti-mouse antibody was used as the secondary.
The antibody was used to block the conversion of prekallikrein to kallikrein in vivo and in vitro (PMID: 17259996). The antibody also reacts with rat retina and vitreous.
This image is courtesy of an anonymous Abreview
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Plasma Kallikrein 1B antibody [13G11](ab1006)
](/ps/datasheet/images/1/ab1006/Plasma-Kallikrein-1B-Primary-antibodies-ab1006-3.jpg)
ab1006 (4µg/ml) staining plasma kallikrein in human liver, using an automated system (DAKO Autostainer Plus). Using this protocol there is weak cytoplasmic staining of a subpopulation of hepatocytes.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
References for Anti-Plasma Kallikrein 1B antibody [13G11] (ab1006)
This product has been referenced in:
- Liu Jet al. Hyperglycemia-induced cerebral hematoma expansion is mediated by plasma kallikrein. Nat Med 17:206-10 (2011). Functional Studies; Rat.Read more (PubMed: 21258336) »
- Morris KEet al. Autonomic nervous system regulates secretion of anti-inflammatory prohormone SMR1 from rat salivary glands. Am J Physiol Cell Physiol 296:C514-24 (2009). WB; Rat.Read more (PubMed: 19109528) »
See all 7 publications for this product
Publishing research using ab1006? Please let us know so that we can cite the reference in this datasheet
![Prekallikrein / Kallikrein antibody [13G11] for WB in Human (1006)](/ps/Reviews/Images/ab6786_26357.jpg)
![Prekallikrein / Kallikrein antibody [13G11] for WB in Rat (1006)](/ps/Reviews/Images/ab6787_74154.jpg)
![Plasma Kallikrein 1B antibody [13G11] for WB in Human (1006)](/ps/datasheet/images/1/ab1006/Plasma-Kallikrein-1B-Primary-antibodies-ab1006-2.png)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"