Anti-Plasma Kallikrein 1B antibody (ab44392)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab44392 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 71 kDa. 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection.

Target

  • FunctionThe enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.
  • Involvement in diseaseDefects in KLKB1 are the cause of prekallikrein deficiency (PKK deficiency) [MIM:612423]; also known as Fletcher factor deficiency. This disorder is a blood coagulation defect.
  • Sequence similaritiesBelongs to the peptidase S1 family. Plasma kallikrein subfamily.
    Contains 4 apple domains.
    Contains 1 peptidase S1 domain.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Fletcher factor antibody
    • kallikrein B plasma antibody
    • Kallikrein B plasma (Fletcher factor) 1 antibody
    • Kallikrein B, plasma 1 antibody
    • Kininogenin antibody
    • KLK3 antibody
    • KLKB1 antibody
    • KLKB1_HUMAN antibody
    • Plasma kallikrein antibody
    • Plasma kallikrein B1 antibody
    • Plasma kallikrein light chain antibody
    • Plasma prekallikrein antibody
    • PPK antibody
    • Prekallikrein antibody
    see all

Anti-Plasma Kallikrein 1B antibody images

  • ab44392 staining Kallikrein 1B in human temporal neocortical tissue sections IHC-P . Tissue samples were fixed with formaldehyde and blocked with 10% serum for 20 minutes at 23°C: antigen retrieval was performed by heat mediation in citrate buffer.. Samples were incubated with primary antibody (1/100) for 18 hours at 23°C . A biotin-conjugated horse anti Rabbit IgG  polyclonal (1/50) was used as secondary antibody.

    See Abreview

References for Anti-Plasma Kallikrein 1B antibody (ab44392)

ab44392 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Pig Purified protein (porcine plasma, porcine articular capsule extract)
Gel Running Conditions Non-reduced Denaturing (10% PAGE)
Loading amount 0.2 µg
Specification porcine plasma, porcine articular capsule extract
Blocking step Tween 20 as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: 20°C
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Abcam user community

Verified customer

Submitted Feb 10 2016

I will send the alignment results for ab116517, hopefully later today.

I am glad ab1006 is working, at least with human samples. If you have time, we encourage our customers to send us reviews of their results, positive or negative, via our ...

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This is frustrating for us too, finding incorrect information on our datasheets. We try to minimize it but there is clearly work to be done.

I did not realize you had already purchased the antibody. If you would still like to try it with rat...

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My apologies: the laboratory that supplies this to us confirmed that they have not tested this for reactivity to rat protein, for have we or, to our knowledge, any of our customers. It is not clear why our datasheet lists rat in the "Reacts With" field...

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Thank you for contacting us. Unfortunately, the immunogen sequence for that antibody is in fact proprietary, and we do not offer or accept confidentiality agreements, for this or other immunogen sequences.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application ELISA
Sample Human Cell (human brain)
Specification human brain
Blocking step Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 0.5% · Temperature: 22°C
Type Sandwich (Detection)
Username

Miss. Emma Ashby

Verified customer

Submitted Dec 10 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (temporal neocortex)
Specification temporal neocortex
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization No
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Nov 26 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Purified protein (human plasma)
Loading amount 4 µg
Specification human plasma
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Oct 26 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (blood)
Loading amount 20 µg
Specification blood
Gel Running Conditions Reduced Denaturing (4-20% gel)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jun 16 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"