Anti-Plasma Kallikrein 1B antibody [EPR5707] (ab124938)

Overview

  • Product nameAnti-Plasma Kallikrein 1B antibody [EPR5707]
    See all Plasma Kallikrein 1B primary antibodies
  • Description
    Rabbit monoclonal [EPR5707] to Plasma Kallikrein 1B
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to residues in the C terminus of Human Plasma Kallikrein 1B.

  • Positive control
    • Human breast cancer, Human plasma, Human ovary and fetal liver lysates; Human kidney tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab124938 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 71 kDa).
IP 1/10 - 1/100.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionThe enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.
  • Involvement in diseaseDefects in KLKB1 are the cause of prekallikrein deficiency (PKK deficiency) [MIM:612423]; also known as Fletcher factor deficiency. This disorder is a blood coagulation defect.
  • Sequence similaritiesBelongs to the peptidase S1 family. Plasma kallikrein subfamily.
    Contains 4 apple domains.
    Contains 1 peptidase S1 domain.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Fletcher factor antibody
    • kallikrein B plasma antibody
    • Kallikrein B plasma (Fletcher factor) 1 antibody
    • Kallikrein B, plasma 1 antibody
    • Kininogenin antibody
    • KLK3 antibody
    • KLKB1 antibody
    • KLKB1_HUMAN antibody
    • Plasma kallikrein antibody
    • Plasma kallikrein B1 antibody
    • Plasma kallikrein light chain antibody
    • Plasma prekallikrein antibody
    • PPK antibody
    • Prekallikrein antibody
    see all

Anti-Plasma Kallikrein 1B antibody [EPR5707] images

  • ab124938, at 1/50 dilution, staining Plasma Kallikrein 1B in paraffin-embedded Human kidney tissue by Immunohistochemistry.
  • All lanes : Anti-Plasma Kallikrein 1B antibody [EPR5707] (ab124938) at 1/1000 dilution

    Lane 1 : Human breast cancer lysate
    Lane 2 : Human plasma lysate
    Lane 3 : Human ovary lysate
    Lane 4 : Fetal liver lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP labelled Goat anti Rabbit IgG at 1/2000 dilution

    Predicted band size : 71 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)
  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

References for Anti-Plasma Kallikrein 1B antibody [EPR5707] (ab124938)

ab124938 has not yet been referenced specifically in any publications.

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Ab124938 is provided as unpurified tissue culture supernatant, and since there other proteins present in this solution, it isn't possible to measure the concentration of the specific antibody. S...

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