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Full length native protein (purified) (Human).
Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.
Our Abpromise guarantee covers the use of ab10178 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|AP||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration. Antigen retrieval is not essential but may optimise staining.|
|Flow Cyt||Use 2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|Other||Use at an assay dependent concentration.|
ab10178 staining human brain tissue sections by IHC-P. Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in EDTA prior to blocking in 10% serum for 20 hours at 37°C. The primary antibody was diluted 1/200 and incubated with the sample for 18 hours at 37°C. A biotinylated horse anti-mouse IgG antibody, diluted 1/500, was used as the secondary.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"