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Western blot - Plastin L antibody (ab81454)
(enlarge)
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Product Name
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Plastin L antibody
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See all Plastin L antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Plastin L
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Immunogen
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Synthetic peptide: LNILEEIGGG QKVNDDIIVN WVNETLREAK KSSSISSFKD PKISTSLPVL, corresponding to a region within internal sequence amino acids 504 - 553 of Human Plastin L (NP_002289)
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at an assay dependent dilution.
ELISA titre using peptide based assay: 1/62500
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 70 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Placenta lysate
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Cellular localization
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Cytoplasmic
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Research areas
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Cancer >> Tumor biomarkers >> Tumor antigens
Cancer >> Tumor immunology >> Tumor-associated antigens
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Binding Proteins
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Relevance
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Lipoprotein lipase (LPL) is the central enzyme in plasma triglyceride hydrolysis and is secreted by macrophages in the subendothelial space. Evidence has been provided that LPL produced by macrophages in the vessel wall exerts proatherogenic effects. The atherogenic effects of LPL have been mainly attributed to its ability to favor lipid accumulation within macrophages present in the atherosclerotic lesion. Recently, it has also been shown that LPL promote the development of atherosclerosis through facilitation of monocyte adhesion to endothelial cells, stimulation of tumor necrosis factor alpha (TNF ) secretion and induction of vascular smooth muscle cell proliferation.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: None
Constituents: 2% Sucrose, PBS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Plastin L antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Plastin L antibody images, references, products related to ab81454 and other tools.
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Plastin L antibody images:
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 Western blot - Plastin L antibody (ab81454)
Plastin L antibody (ab81454) at 1 µg/ml (in 5% skim milk / PBS buffer) + placenta lysate at 10 µg/ml
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 70 kDa
Observed band size : 64 kDa (why is the actual band size different from the predicted?)
Gel concentration 12%
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Search PubMed (MEDLINE) for references to Plastin L
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Plastin L antibody - more information
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Customer reviews (feedback) regarding Plastin L antibody |
Customer FAQs regarding Plastin L antibody |
Protocols for Plastin L antibody |
Price and availability of products related to Plastin L antibody |
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Plastin L antibody - related products:
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Plastin L antibody - other tools:
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Contact Abcam with a Technical Enquiry about Plastin L antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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