Recombinant
RabMAb

Anti-Plastin L antibody [EPR4277] (ab109124)

Overview

  • Product name
    Anti-Plastin L antibody [EPR4277]
    See all Plastin L primary antibodies
  • Description
    Rabbit monoclonal [EPR4277] to Plastin L
  • Tested applications
    Suitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human Plastin L.

  • Positive control
    • THP1, K562 and Jurkat cell lysates; Human tonsil tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab109124 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 70 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C allow to cool for 10-20 min).
ICC 1/250 - 1/500.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      Actin-binding protein. Plays a role in the activation of T-cells in response to costimulation through TCR/CD3 and CD2 or CD28. Modulates the cell surface expression of IL2RA/CD25 and CD69.
    • Tissue specificity
      Detected in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia, in spleen and other lymph node-containing organs. Expressed in peripheral blood T lymphocytes, neutrophils, monocytes, B lymphocytes, and myeloid cells.
    • Sequence similarities
      Contains 2 actin-binding domains.
      Contains 4 CH (calponin-homology) domains.
      Contains 2 EF-hand domains.
    • Post-translational
      modifications
      Phosphorylated on a serine residue in response to costimulation through TCR/CD3 and CD2 or CD28. Serine phosphorylation promotes association with the actin cytoskeleton and targeting to peripheral cell projections.
      The N-terminus is blocked.
    • Cellular localization
      Cytoplasm > cytoskeleton. Cell junction. Cell projection. Cell projection > ruffle membrane. Relocalizes to the immunological synapse between peripheral blood T lymphocytes and antibody-presenting cells in response to costimulation through TCR/CD3 and CD2 or CD28. Associated with the actin cytoskeleton at membrane ruffles (By similarity). Relocalizes to actin-rich cell projections upon serine phosphorylation.
    • Information by UniProt
    • Database links
    • Alternative names
      • CP64 antibody
      • L plastin antibody
      • L-plastin antibody
      • Larval cuticle protein 1 antibody
      • LC64P antibody
      • LCP-1 antibody
      • LCP1 antibody
      • LPL antibody
      • Lplastin antibody
      • Lymphocyte cytosolic protein 1 antibody
      • Plastin 2 antibody
      • Plastin-2 antibody
      • PLS2 antibody
      • PLSL_HUMAN antibody
      see all

    Images

    • All lanes : Anti-Plastin L antibody [EPR4277] (ab109124) at 1/1000 dilution

      Lane 1 : THP1 cell lysates
      Lane 2 : K562 cell lysates
      Lane 3 : Jurkat cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled Goat anti-Rabbit at 1/2000 dilution

      Predicted band size : 70 kDa
    • ab109124, at 1/100 dilution staining Plastin L in paraffin-embedded Human tonsil tissue, by Immunohistochemistry.

    References

    ab109124 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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