Recombinant Anti-Plastin L antibody [EPR4278] (ab109129)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4278] to Plastin L
- Suitable for: WB, IHC-P, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Plastin L antibody [EPR4278]
See all Plastin L primary antibodies -
Description
Rabbit monoclonal [EPR4278] to Plastin L -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: THP-1, Jurkat, and K562 cell lysates, IHC-P: Human tonsil tissue; Flow Cyt (intra): MDA-MB-435S cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4278 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109129 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/10000 - 1/50000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Flow Cyt (Intra) |
1/1000.
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Notes |
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WB
1/10000 - 1/50000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa). |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Flow Cyt (Intra)
1/1000. |
Target
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Function
Actin-binding protein. Plays a role in the activation of T-cells in response to costimulation through TCR/CD3 and CD2 or CD28. Modulates the cell surface expression of IL2RA/CD25 and CD69. -
Tissue specificity
Detected in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia, in spleen and other lymph node-containing organs. Expressed in peripheral blood T lymphocytes, neutrophils, monocytes, B lymphocytes, and myeloid cells. -
Sequence similarities
Contains 2 actin-binding domains.
Contains 4 CH (calponin-homology) domains.
Contains 2 EF-hand domains. -
Post-translational
modificationsPhosphorylated on a serine residue in response to costimulation through TCR/CD3 and CD2 or CD28. Serine phosphorylation promotes association with the actin cytoskeleton and targeting to peripheral cell projections.
The N-terminus is blocked. -
Cellular localization
Cytoplasm > cytoskeleton. Cell junction. Cell projection. Cell projection > ruffle membrane. Relocalizes to the immunological synapse between peripheral blood T lymphocytes and antibody-presenting cells in response to costimulation through TCR/CD3 and CD2 or CD28. Associated with the actin cytoskeleton at membrane ruffles (By similarity). Relocalizes to actin-rich cell projections upon serine phosphorylation. - Information by UniProt
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Database links
- Entrez Gene: 3936 Human
- Omim: 153430 Human
- SwissProt: P13796 Human
- Unigene: 381099 Human
- Unigene: 710563 Human
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Alternative names
- CP64 antibody
- L plastin antibody
- L-plastin antibody
see all
Images
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Immunohistochemical staining of paraffin-embedded Human tonsil tissue using ab109129 at a dilution of 1/250.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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All lanes : Anti-Plastin L antibody [EPR4278] (ab109129) at 1/10000 dilution
Lane 1 : THP-1 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 70 kDa -
Flow Cytometry analysis of MDA-MB-435S (Human mammary gland ductal carcinoma melanocyte) cells labeling Plastin L with purified ab109129 at 1/1000 dilution (1 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab109129 has been referenced in 2 publications.
- Shapanis A et al. Identification of proteins associated with development of metastasis from cutaneous squamous cell carcinomas (cSCCs) via proteomic analysis of primary cSCCs. Br J Dermatol 184:709-721 (2021). PubMed: 32794257
- Chen C et al. AP4 modulated by the PI3K/AKT pathway promotes prostate cancer proliferation and metastasis of prostate cancer via upregulating L-plastin. Cell Death Dis 8:e3060 (2017). WB ; Human . PubMed: 28981098