Anti-Plastin L antibody [EPR4278] (ab109129)

Overview

  • Product nameAnti-Plastin L antibody [EPR4278]
    See all Plastin L primary antibodies
  • Description
    Rabbit monoclonal [EPR4278] to Plastin L
  • Tested applicationsSuitable for: WB, IP, IHC-P, ICC, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human Plastin L.

  • Positive control
    • THP-1, Jurkat, and K562 cell lysates, Human tonsil tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab109129 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/500 - 1/1000.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • FunctionActin-binding protein. Plays a role in the activation of T-cells in response to costimulation through TCR/CD3 and CD2 or CD28. Modulates the cell surface expression of IL2RA/CD25 and CD69.
  • Tissue specificityDetected in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia, in spleen and other lymph node-containing organs. Expressed in peripheral blood T lymphocytes, neutrophils, monocytes, B lymphocytes, and myeloid cells.
  • Sequence similaritiesContains 2 actin-binding domains.
    Contains 4 CH (calponin-homology) domains.
    Contains 2 EF-hand domains.
  • Post-translational
    modifications
    Phosphorylated on a serine residue in response to costimulation through TCR/CD3 and CD2 or CD28. Serine phosphorylation promotes association with the actin cytoskeleton and targeting to peripheral cell projections.
    The N-terminus is blocked.
  • Cellular localizationCytoplasm > cytoskeleton. Cell junction. Cell projection. Cell projection > ruffle membrane. Relocalizes to the immunological synapse between peripheral blood T lymphocytes and antibody-presenting cells in response to costimulation through TCR/CD3 and CD2 or CD28. Associated with the actin cytoskeleton at membrane ruffles (By similarity). Relocalizes to actin-rich cell projections upon serine phosphorylation.
  • Information by UniProt
  • Database links
  • Alternative names
    • CP64 antibody
    • L plastin antibody
    • L-plastin antibody
    • Larval cuticle protein 1 antibody
    • LC64P antibody
    • LCP-1 antibody
    • LCP1 antibody
    • LPL antibody
    • Lplastin antibody
    • Lymphocyte cytosolic protein 1 antibody
    • Plastin 2 antibody
    • Plastin-2 antibody
    • PLS2 antibody
    • PLSL_HUMAN antibody
    see all

Anti-Plastin L antibody [EPR4278] images

  • Immunohistochemical staining of paraffin-embedded Human tonsil tissue using ab109129 at a dilution of 1/250.
  • All lanes : Anti-Plastin L antibody [EPR4278] (ab109129) at 1/10000 dilution

    Lane 1 : THP-1 cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : K562 cell lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 70 kDa

References for Anti-Plastin L antibody [EPR4278] (ab109129)

ab109129 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"